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Alterations in p53 and E2F-1 function common to immortalized chicken embryo fibroblasts

Oncogene volume 20, pages 2671–2682 (2001)Cite this article

Abstract

A number of non-virally and non-chemically immortalized chicken embryo fibroblast (CEF) cells have been established recently in continuous cell culture. All immortal CEF cells tested showed common genetic alterations in the expression patterns of p53 and E2F-1 mRNA and protein which were down- and up-regulated, respectively. The biological effects of differentially regulated p53 and E2F-1 were determined by reporter gene transcriptional activity assays, DNA binding assays, and Northern blot analysis of the expression patterns of down-stream genes. In addition, expression of most of the cyclin genes was up-regulated in immortal CEF cells, which may be associated with the rapid cell division rates and serum-independent growth patterns seen in immortal CEF cells. The telomeric lengths and chromosome integrity were maintained in all immortal CEF cell lines without detectable telomerase activity. Although the functional inactivations of the p53 and Rb regulatory pathways are known to be common events for cellular immortalization, the genetic changes leading to alteration of p53 and E2F-1 function through transcriptional and post-transcriptional regulation seem to be unique in immortal CEF cells.

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Acknowledgements

We thank Dr Charles J Sherr (St Jude Children's Research Hospital) for providing the chicken cyclin cDNAs. This work was supported, in part, by USDA/NRICGP grant #9603280 and a grant from American Home Products (Fort Dodge Animal Health) to DN Foster.

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  1. In-Jeong Kim

    Present address: Trudeau Institute, PO Box 59, 100 Algonquin Avenue, Saranac Lake, 12983, NY, USA

Authors and Affiliations

  1. Department of Animal Science, University of Minnesota, St. Paul, 55108, Minnesota, MN, USA

    Hyunggee Kim, Linda K Foster, James Farris, Sakthikumar Ambady, F Abel Ponce de León & Douglas N Foster

  2. Veterinary PathoBiology, University of Minnesota, St. Paul, 55108, Minnesota, MN, USA

    In-Jeong Kim

  3. School of Agricultural Biotechnology, Seoul National University, Suwon, 441-744, Korea

    Seungkwon You

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Kim, H., You, S., Kim, IJ. et al. Alterations in p53 and E2F-1 function common to immortalized chicken embryo fibroblasts. Oncogene 20, 2671–2682 (2001). https://doi.org/10.1038/sj.onc.1204378

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