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  • Original Paper
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E2Fs up-regulate expression of genes involved in DNA replication, DNA repair and mitosis

Oncogene volume 21, pages 437–446 (2002)Cite this article

Abstract

The E2F family of transcription factors plays a pivotal role in the regulation of cell proliferation in higher eukaryotes. We used DNA microarrays and cell lines containing either inducible E2F-1 or inducible E2F-3 to identify novel E2F target genes. Our data indicate that E2F up-regulates the expression of genes not previously described as E2F target genes. A number of these E2F-regulated genes are involved in DNA replication, DNA repair and mitosis. These results suggest that E2F affects cell cycle progression both at S phase and during mitosis. Furthermore, our findings indicate that E2F-dependent gene activation may contribute to the cellular response to DNA damage.

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Acknowledgements

We thank Drs WG Kaelin Jr and PD Adams for Rat-1-MT-E2F-1 cells, Dr Shirley Horn-Saban for excellent technical assistance with DNA microarray analysis, Dr Ron Ophir for assistance with the statistical analysis of the data and Gili Hart for technical assistance. This work was supported in part by grants from the Israel Cancer Research Fund (ICRF), Minerva Foundation (Germany) and Yad Abraham Research Center for Diagnostics and Therapy. D Ginsberg is an incumbent of the Recanati Career Development chair of cancer research.

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  1. Shirley Polager and Yael Kalma: These authors contributed equally to this research

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  1. Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, Israel

    Shirley Polager, Yael Kalma, Eli Berkovich & Doron Ginsberg

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  1. Shirley Polager
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  2. Yael Kalma
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Correspondence to Doron Ginsberg.

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Polager, S., Kalma, Y., Berkovich, E. et al. E2Fs up-regulate expression of genes involved in DNA replication, DNA repair and mitosis. Oncogene 21, 437–446 (2002). https://doi.org/10.1038/sj.onc.1205102

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