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  • Original Paper
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The putative tumor suppressor RASSF1A homodimerizes and heterodimerizes with the Ras-GTP binding protein Nore1

Oncogene volume 21, pages 1381–1390 (2002)Cite this article

A Corrigendum to this article was published on 13 March 2002

Abstract

Nore and RASSF1A are noncatalytic proteins that share 50% identity over their carboxyterminal 300 AA, a segment that encompasses a putative Ras-Rap association (RA) domain. RASSF1 is expressed as several splice variants, each of which contain an RA domain, however the 340 AA RASSF1A, but not the shorter RASSF1C variant, is a putative tumor suppressor. Nore binds to Ras and several Ras-like GTPases in a GTP dependent fashion however neither RASSF1 (A or C) or the C. elegans Nore/RASSF1 homolog, T24F1.3 exhibit any interaction with Ras or six other Ras-like GTPases in a yeast two-hybrid expression assay. A low recovery of RASSF1A (but not RASSF1C) in association with RasG12V is observed however on transient expression in COS cells. Nore and RASSF1A can each efficiently homodimerize and heterodimerize with each other through their nonhomologous aminoterminal segments. Recombinant RASSF1C exhibits a much weaker ability to homodimerize or heterodimerize; thus the binding of RASSF1C to Nore is very much less than the binding of RASSF1A to Nore. The association of RASSF1A with RasG12V in COS cells appears to reflect the heterodimerization of RASSF1A with Nore, inasmuch the recovery of RASSF1A with RasG12V is increased by concurrent expression of full length Nore, and abolished by expression of Nore deleted of its RA domain. The preferential ability of RASSF1A to heterodimerize with Nore and thereby associate with Ras-like GTPases may be relevant to its putative tumor suppressor function.

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Acknowledgements

We thank Lawrence Quilliam for M-Ras constructs, and Christian Herrmann for Rap 2A, TC21 and R-Ras. Chris Ponting provided helpful discussion. S Ortiz-Vega is supported by NIH T32DK07028. A Khokhlatchev is supported by the Leukemia and Lymphoma Society of America. GP Pfeifer was supported by a grant from the University of California Tobacco Related Disease Research Program (9RT-0175).

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Author notes

  1. Sara Ortiz-Vega and Andrei Khokhlatchev: S Ortiz-Vega and A Khokhlatchev contributed equally to this work

Authors and Affiliations

  1. Diabetes Unit and Medical Services, Massachusetts General Hospital, Boston, 02114, Massachusetts, MA, USA

    Sara Ortiz-Vega, Andrei Khokhlatchev, Maria Nedwidek, Xian-feng Zhang & Joseph Avruch

  2. Department of Molecular Biology, Massachusetts General Hospital, Boston, 02114, Massachusetts, MA, USA

    Sara Ortiz-Vega & Andrei Khokhlatchev

  3. Department of Medicine, Harvard Medical School, Boston, 02114, MA, USA

    Sara Ortiz-Vega & Andrei Khokhlatchev

  4. Beckman Research Institute, City of Hope, 1500 E. Duarte Rd, Duarte, California, 91010, CA, USA

    Reinhard Dammann & Gerd P Pfeifer

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  1. Sara Ortiz-Vega
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Correspondence to Joseph Avruch.

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Ortiz-Vega, S., Khokhlatchev, A., Nedwidek, M. et al. The putative tumor suppressor RASSF1A homodimerizes and heterodimerizes with the Ras-GTP binding protein Nore1. Oncogene 21, 1381–1390 (2002). https://doi.org/10.1038/sj.onc.1205192

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