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Genes involved in cell adhesion, cell motility and mitogenic signaling are altered due to HPV 16 E5 protein expression

Oncogene volume 27, pages 2532–2541 (2008)Cite this article

Abstract

We investigated the effects of the human papillomavirus type 16 E5 oncogene on cellular gene expression in human epithelial cells using cDNA microarray. In a genome-wide microarray assay, the expression of 179 genes was found to be significantly altered due to E5 expression. The expression of lamin A/C was downregulated at protein level. The expression of protein kinase C-δ and phosphoinositide-3-kinase proteins was found to be upregulated. We also observed increased motility of E5-expressing cells. We conclude that the E5 protein affects several cellular pathways involved in cell adhesion, cell motility and mitogenic signaling. These alterations may together lead to inhibition of apoptosis and facilitate the establishment of persistent infection in the epithelium.

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Acknowledgements

We thank Professor Angel Alonso (DKFZ, Heidelberg, Germany) for providing the HaCaT-E5 and -pMSG cell lines. We acknowledge Rita Fingerroos for excellent technical assistance and Anu Planken for assistance in PCR technology. Light Microscopy Unit (Institute of Biotechnology, University of Helsinki, Finland) is acknowledged for their support in cell motility assays. N Kivi was supported by grants from the University of Helsinki Research Foundation, the Oskar Öflund Foundation, the Biomedicum Helsinki Foundation, Fund of Laboratory Medicine Research and the K Albin Johansson Foundation.

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  1. Department of Virology, Haartman Institute, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland

    N Kivi & E Auvinen

  2. Institute of Biotechnology, University of Helsinki, Helsinki, Finland

    D Greco & P Auvinen

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Correspondence to N Kivi.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Kivi, N., Greco, D., Auvinen, P. et al. Genes involved in cell adhesion, cell motility and mitogenic signaling are altered due to HPV 16 E5 protein expression. Oncogene 27, 2532–2541 (2008). https://doi.org/10.1038/sj.onc.1210916

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