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Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations

Nature volume 306, pages 658–661 (1983)Cite this article

Abstract

Each of nine mammary carcinomas induced by a single injection of nitroso-methylurea into 50-day-old Buf/N female rats, contained a transforming H-ras-1 gene. Molecular characterization of one of the genes revealed that the twelfth codon was GAA instead of GGA of the normal allele, encoding glutamic acid in place of glycine. These results indicate that chemical carcinogenesis represents an adequate model to study the role of transforming ras genes in human neoplasia.

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Authors and Affiliations

  1. Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland, 20205, USA

    Saraswati Sukumar, Vicente Notario, Dionisio Martin-Zanca & Mariano Barbacid

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  1. Saraswati Sukumar
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  2. Vicente Notario
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  3. Dionisio Martin-Zanca
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  4. Mariano Barbacid
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Sukumar, S., Notario, V., Martin-Zanca, D. et al. Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations. Nature 306, 658–661 (1983). https://doi.org/10.1038/306658a0

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