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  • Original Paper
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Xmeis1, a protooncogene involved in specifying neural crest cell fate in Xenopus embryos

Oncogene volume 20, pages 1329–1342 (2001)Cite this article

Abstract

Meis1 (Myeloid Ecotropic viral Integration Site 1) is a homeobox gene that was originally isolated as a common site of viral integration in myeloid tumors of the BXH-2 recombinant inbred mice strain. We previously isolated a Xenopus homolog of Meis1 (Xmeis1). Here we show that Xmeis1 may play a significant role in neural crest development. In developing Xenopus embryos, Xmeis1 displays a broad expression pattern, but strong expression is observed in tissue of neural cell fate, such as midbrain, hindbrain, the dorsal portion of the neural tube, and neural crest derived branchial arches. In animal cap explants, overexpression of Xmeis1b, an alternatively spliced form of Xmeis1, induces expression of neural crest marker genes in the absence of mesoderm. Moreover, Xmeis1b induces XGli-3 and XZic3, pre-pattern genes involved at the earliest stages of neural crest development, and like these two genes, can induce ectopic pigmented cell masses when overexpressed in developing embryos. Misexpression of Xmeis1b also induces ectopic expression of neural crest markers along the antero-posterior axis of the neural tube in developing Xenopus embryos. In contrast, Xmeis1a, another splice variant, is much less effective at inducing these effects. These data suggest that Xmeis1b is involved in neural crest cell fate specification during embryogenesis, and can functionally intersect with the Gli/Zic signal transduction pathway.

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Acknowledgements

We would like to thank Andy Golden, Lino Tessarollo, and Nancy Papalopulu for critical reading of the manuscript, and thank David Wilkinson (Krox-20), Igor Dawid (N-tubulin and eFGF), Thomas Sargent (XAP-2, XK81), Masazumi Tada (pSP64T-GR construct) for cDNAs. Activin A was obtained through NHPP, NIDDK and Dr Parlow. This project has been funded in whole or in part with funds from the National Cancer Institute, National Institute of Health, under contract No. NO1-CO-5600. R Maeda was supported by a JSPS Research Fellowship for Japanese Biomedical and Behavioral Researchers.

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  1. Teri L Jones

    Present address: Kirkegaard and Perry Laboratories, 2 Cessna Court, Gaithersburg, 20879-4174, Maryland, MD, USA

Authors and Affiliations

  1. Regulation of Cell Growth Laboratory, National Cancer Institute–Frederick, Frederick, 21702, Maryland, MD, USA

    Ryu Maeda, Kathleen Mood, Teri L Jones & Ira O Daar

  2. Laboratory of Developmental Neurobiology, Brain Science Institute, RIKEN, Wako-shi, 351-0198, Saitama, Japan

    Jun Aruga

  3. Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, 19107, Pennsylvania, PA, USA

    Arthur M Buchberg

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Maeda, R., Mood, K., Jones, T. et al. Xmeis1, a protooncogene involved in specifying neural crest cell fate in Xenopus embryos. Oncogene 20, 1329–1342 (2001). https://doi.org/10.1038/sj.onc.1204250

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