Elastic properties of polyunsaturated phosphatidylethanolamines influence rhodopsin function

Walter E. Teague; Olivier Soubias; Horia Petrache; Nola Fuller; Kirk G. Hines; R. Peter Rand; Klaus Gawrisch

doi:10.1039/C2FD20095C

Elastic properties of polyunsaturated phosphatidylethanolamines influence rhodopsin function

Walter E. Teague, Jr.,^a Olivier Soubias,^a Horia Petrache,^b Nola Fuller,^c Kirk G. Hines,^a R. Peter Rand^c and Klaus Gawrisch*^a

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* Corresponding authors

^a Laboratory of Membrane Biochemistry and Biophysics, NIAAA, NIH, Bethesda, MD 20892, USA
E-mail: gawrisch@helix.nih.gov
Fax: +1-301-594-0035
Tel: +1-301-594-3750

^b Department of Physics, Indiana University–Perdue University, Indianapolis, IN 46202, USA
E-mail: hpetrach@iupui.edu
Fax: +1-317-274-2392
Tel: +1-317-278-6521

^c Department of Biological Science, Brock University, St. Catharines, Ont. L2S 3A1, Canada
E-mail: rrand@brocku.ca
Tel: +1-905-688-5550 ext.3388

Abstract

Membranes with a high content of polyunsaturated phosphatidylethanolamines (PE) facilitate formation of metarhodopsin-II (M_II), the photointermediate of bovine rhodopsin that activates the G protein transducin. We determined whether M_II-formation is quantitatively linked to the elastic properties of PEs. Curvature elasticity of monolayers of the polyunsaturated lipids 18 : 0–22 : 6_n − 3PE, 18 : 0–22 : 5_n − 6PE and the model lipid 18 : 1_n − 9–18 : 1_n − 9PE were investigated in the inverse hexagonal phase. All three lipids form lipid monolayers with rather low spontaneous radii of curvature of 26–28 Å. In membranes, all three PEs generate high negative curvature elastic stress that shifts the equilibrium of M_I/M_II photointermediates of rhodopsin towards M_II formation.

This article is part of the themed collection: Lipids & Membrane Biophysics

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https://doi.org/10.1039/C2FD20095C

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Paper

Submitted

02 May 2012

Accepted

28 Jun 2012

First published

29 Jun 2012

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Faraday Discuss., 2013,161, 383-395

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Elastic properties of polyunsaturated phosphatidylethanolamines influence rhodopsin function

W. E. Teague, O. Soubias, H. Petrache, N. Fuller, K. G. Hines, R. P. Rand and K. Gawrisch, Faraday Discuss., 2013, 161, 383 DOI: 10.1039/C2FD20095C

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Elastic properties of polyunsaturated phosphatidylethanolamines influence rhodopsin function

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