Volume 161, 2013
From the journal:

Faraday Discussions

Gibbs energy determinants of lipoprotein insertion into lipid membranes: the case study of Ras proteins

Katrin Weise,a   Daniel Huster,b   Shobhna Kapoor,a   Gemma Triola,c   Herbert Waldmannc  and  Roland Winter*a  

* Corresponding authors

a Physical Chemistry I, Faculty of Chemistry, TU Dortmund University, Dortmund, Germany
E-mail: roland.winter@tu-dortmund.de
Fax: +49 (0) 231 755 3901
Tel: +49 (0) 231 755 3900

b Institute of Medical Physics and Biophysics, University of Leipzig, Leipzig, Germany

c Chemical Biology, Max Planck Institute of Molecular Physiology, Dortmund, Faculty of Chemistry, TU Dortmund University, Dortmund, Germany

Abstract

In a combined chemical-biological and biophysical approach we explored the Gibbs (free) energy contributions to the membrane partitioning of lipidated proteins, and compared the theoretical predictions with recent experimental data on the membrane insertion of Ras proteins of various anchor systems into rationally designed model biomembrane systems. Various factors fostering or reducing the membrane partitioning properties are discussed, including hydrophobic effects, lipid chain mismatch, electrostatic interactions, membrane-mediated protein–protein interactions, and terms that account for line tension effects between coexisting lipid domains, lipid sorting, and changes in the lateral organization of the lipid bilayer system. From these data, it is apparent that two membrane anchoring motifs are needed to facilitate firm membrane binding. For heterogeneous membranes, localization and sequestration at domain boundaries as well as formation of protein clusters and collective lateral organization via an effective lipid sorting mechanism provide complementary ways of inducing membrane nanodomains that could potentially operate as effective, high fidelity signalling platforms.

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Article information

DOI
https://doi.org/10.1039/C2FD20100C
Article type
Paper
Submitted
05 May 2012
Accepted
25 Jun 2012
First published
25 Jun 2012

Faraday Discuss., 2013,161, 549-561

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Gibbs energy determinants of lipoprotein insertion into lipid membranes: the case study of Ras proteins

K. Weise, D. Huster, S. Kapoor, G. Triola, H. Waldmann and R. Winter, Faraday Discuss., 2013, 161, 549 DOI: 10.1039/C2FD20100C

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