Issue 11, 2005

Gene sensors based on peptide nucleic acid (PNA) probes: Relationship between sensor sensitivity and probe/target duplex stability

Abstract

Gene sensors based on peptide nucleic acid (PNA) probes were prepared and the relationship between sensor sensitivity and the duplex stability of the probe PNAs and target complementary DNAs was studied using five synthesized PNAs (10-, 15-, 17-, 20-, and 22-mers). It was found that the association constants for the probe PNA/target DNA pairs depend not only on the length but also on the base pair sequence, and that the trend in the sensor responses was the same as that in the association constants for the corresponding pairs. In addition, by using two kinds of probe PNAs with different lengths, it was demonstrated that fabrication of sensors based on probe PNAs with comparable association constants yielded similar response curves and sensor sensitivities.

Graphical abstract: Gene sensors based on peptide nucleic acid (PNA) probes: Relationship between sensor sensitivity and probe/target duplex stability

Article information

Article type
Paper
Submitted
19 May 2005
Accepted
05 Jul 2005
First published
02 Aug 2005

Analyst, 2005,130, 1478-1482

Gene sensors based on peptide nucleic acid (PNA) probes: Relationship between sensor sensitivity and probe/target duplex stability

H. Aoki and H. Tao, Analyst, 2005, 130, 1478 DOI: 10.1039/B507121F

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