Issue 2, 2008
From the journal:

Molecular BioSystems

Metabolic flux analysis and metabolic engineering of microorganisms

Hyun Uk Kim,ab   Tae Yong Kimab  and  Sang Yup Lee*abc  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering (BK21 Program), Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea
E-mail: leesy@kaist.ac.kr
Fax: +82 42 869 3910
Tel: +82 42 869 3930

b Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea

c Department of Bio and Brain Engineering, BioProcess Engineering Research Center and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea

Abstract

Recent advances in metabolic flux analysis including genome-scale constraints-based flux analysis and its applications in metabolic engineering are reviewed. Various computational aspects of constraints-based flux analysis including genome-scale stoichiometric models, additional constraints used for the improved accuracy, and several algorithms for identifying the target genes to be manipulated are described. Also, some of the successful applications of metabolic flux analysis in metabolic engineering are reviewed. Finally, we discuss the limitations that need to be overcome to make the results of genome-scale flux analysis more realistically represent the real cell metabolism.

Graphical abstract: Metabolic flux analysis and metabolic engineering of microorganisms

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Article information

DOI
https://doi.org/10.1039/B712395G
Article type
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First published
16 Oct 2007

Mol. BioSyst., 2008,4, 113-120

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Metabolic flux analysis and metabolic engineering of microorganisms

H. U. Kim, T. Y. Kim and S. Y. Lee, Mol. BioSyst., 2008, 4, 113 DOI: 10.1039/B712395G

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