Issue 1, 2008

Enzyme-catalyzed signal amplification for electrochemical DNA detection with a PNA-modified electrode

Abstract

The signal amplification technique of peptide nucleic acid (PNA)-based electrochemical DNA sensor was developed in a label-free and one-step method utilizing enzymatic catalysis. Electrochemical detection of DNAhybridization on a PNA-modified electrode is based on the change of surface charge caused by the hybridization of negatively charged DNA molecules. The negatively charged mediator, ferrocenedicarboxylic acid, cannot diffuse to the DNA hybridized electrode surface due to the charge repulsion with the hybridized DNA molecule while it can easily approach the neutral PNA-modified electrode surface without the hybridization. By employing glucose oxidase catalysis on this PNA-based electrochemical system, the oxidized mediator could be immediately reduced leading to greatly increased electrochemical signals. Using the enzymatic strategy, we successfully demonstrated its clinical utility by detecting one of the mutation sequences of the breast cancer susceptibility gene BRCA1 at a sample concentration lower than 10–9 M. Furthermore, a single base-mismatched sample could be also discriminated from a perfectly matched sample.

Graphical abstract: Enzyme-catalyzed signal amplification for electrochemical DNA detection with a PNA-modified electrode

Article information

Article type
Paper
Submitted
16 Aug 2007
Accepted
08 Oct 2007
First published
16 Oct 2007

Analyst, 2008,133, 100-104

Enzyme-catalyzed signal amplification for electrochemical DNA detection with a PNA-modified electrode

B. Y. Won, H. C. Yoon and H. G. Park, Analyst, 2008, 133, 100 DOI: 10.1039/B712638G

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