Issue 24, 2008

Rapid typing of STRs in the human genome by HyBeacon® melting

Abstract

A new method based on DNA melting has been developed for the rapid analysis of STRs in the human genome. The system is based on homogeneous PCR followed by fluorescence melting analysis and utilises a HyBeacon® probe combined with a PCR primer-blocker oligonucleotide. The use of blockers of different length permits identification of the full range of common D16S539 repeats enabling detection of 99.8% of known alleles. The interrogation of STRs can be carried out on standard genetic analysis platforms and could be applied to other loci to form the basis of a bespoke high-throughput system for use in forensic analysis, particularly as fluorescent genetic analysis platforms are now available for high-resolution melting. This methodology may be suitable for rapid forensic DNA analysis at the point-of-arrest or in a custody suite where it is important to identify an individual from a small group of suspects/detainees.

Graphical abstract: Rapid typing of STRs in the human genome by HyBeacon® melting

Supplementary files

Article information

Article type
Paper
Submitted
04 Aug 2008
Accepted
12 Sep 2008
First published
30 Oct 2008

Org. Biomol. Chem., 2008,6, 4553-4559

Rapid typing of STRs in the human genome by HyBeacon® melting

N. Gale, D. J. French, R. L. Howard, D. G. McDowell, P. G. Debenham and T. Brown, Org. Biomol. Chem., 2008, 6, 4553 DOI: 10.1039/B813431F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements