Issue 23, 2009
From the journal:

Lab on a Chip

Towards an electrowetting-based digital microfluidic platform for magnetic immunoassays†

Vincent Schaller,*a   Anke Sanz-Velasco,a   Alexey Kalabukhov,a   Justin F. Schneiderman,a   Fredrik Öisjöen,a   Aldo Jesorka,b   Andrea Prieto Astalan,c   Anatol Krozer,c   Cristina Rusu,c   Peter Enokssona  and  Dag Winklera  

* Corresponding authors

a Department of Microtechnology and Nanoscience – MC2, Chalmers University of Technology, Göteborg, Sweden
E-mail: vincent.schaller@chalmers.se
Fax: +46 (0)31 772 3622
Tel: +46 (0)31 772 1859

b Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden

c The Imego Institute, Arvid Hedvalls Backe 4, Göteborg, Sweden

Abstract

We demonstrate ElectroWetting-On-Dielectric (EWOD) transport and SQUID gradiometer detection of magnetic nanoparticles (MNPs) suspended in a 2 µl de-ionized water droplet. This proof-of-concept methodology constitutes the first development step towards a highly sensitive magnetic immunoassay platform with SQUID readout and droplet-based sample handling. Magnetic AC-susceptibility measurements were performed on MNPs with a hydrodynamic diameter of 100 nm using a high-Tc dc Superconducting Quantum Interference Device (SQUID) gradiometer as detector. We observed that the signal amplitude per unit volume is 2.5 times higher for a 2 µl sample droplet compared to a 30 µl sample volume.

Graphical abstract: Towards an electrowetting-based digital microfluidic platform for magnetic immunoassays

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Article information

DOI
https://doi.org/10.1039/B912646E
Article type
Technical Note
Submitted
26 Jun 2009
Accepted
07 Sep 2009
First published
01 Oct 2009

Lab Chip, 2009,9, 3433-3436

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Towards an electrowetting-based digital microfluidic platform for magnetic immunoassays

V. Schaller, A. Sanz-Velasco, A. Kalabukhov, J. F. Schneiderman, F. Öisjöen, A. Jesorka, A. P. Astalan, A. Krozer, C. Rusu, P. Enoksson and D. Winkler, Lab Chip, 2009, 9, 3433 DOI: 10.1039/B912646E

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