Issue 18, 2011
From the journal:

Soft Matter

Culture of mammalian cells on patterned superhydrophilic/superhydrophobic silicon nanowire arrays†

Gaëlle Piret,ab   Elisabeth Galopin,ab   Yannick Coffinier,ab   Rabah Boukherroub,*ab   Dominique Legrandc  and  Christian Slomiannyd  

* Corresponding authors

a Institut de Recherche Interdisciplinaire (IRI, CNRS-USR 3078), Université Lille 1, Parc de la Haute Borne, 50 Avenue de Halley, BP 70478, Villeneuve d'Ascq, France
E-mail: rabah.boukherroub@iri.univ-lille1.fr
Fax: +33 3 62 53 17 01
Tel: +33 3 62 53 17 24

b Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN, UMR CNRS 8520), Cité Scientifique, Avenue Poincaré—BP 60069, Villeneuve d'Ascq, France

c Unité de Glycobiologie Structurale et Fonctionnelle (UMR CNRS 8576), Institut Fédératif de Recherche n° 147, Université Lille 1, Villeneuve d'Ascq cedex, France

d Inserm U1003, Laboratoire de Physiologie Cellulaire, Université Lille 1, Villeneuve d'Ascq cedex, France

Abstract

Interfacing nanowires and living cells is highly interesting in various fields including biomedical implants, biosensors or drug delivery. Vertically aligned silicon nanowire (SiNW) arrays prepared by the stain etching technique were investigated in this study. Chemical modification with octadecyltrichlorosilane (OTS) led to the formation of superhydrophobic SiNW surface with a contact angle around 160°. A micropatterned superhydrophilic/superhydrophobic SiNW surface was fabricated using standard optical lithography techniques. Here we report on Chinese Hamster Ovary K1 (CHO) cell culture on patterned superhydrophilic/superhydrophobic silicon nanowire surfaces. It was found that the cells adhered selectively to the superhydrophilic regions while cell adhesion was almost completely suppressed on the superhydrophobic surface. Transmission electron microscopy analysis showed that the cell cytoplasmic projections penetrate the hydrophilic silicon nanowires layer and coat the nanowires, leading to an intimate surface contact and thus a strong adhesion. On the superhydrophobic surface, the cell cytoplasmic projections remained on the top of wires. The nonfouling of the superhydrophobic SiNW substrate was attributed to a stable Cassie–Baxter state, limiting the contact with the culture medium. Another interesting finding from this study is the corrosion of the superhydrophilic SiNW surface in phosphate-buffered saline (PBS) solution.

Graphical abstract: Culture of mammalian cells on patterned superhydrophilic/superhydrophobic silicon nanowire arrays

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Article information

DOI
https://doi.org/10.1039/C1SM05838J
Article type
Paper
Submitted
06 May 2011
Accepted
27 Jun 2011
First published
02 Aug 2011

Soft Matter, 2011,7, 8642-8649

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Culture of mammalian cells on patterned superhydrophilic/superhydrophobic silicon nanowire arrays

G. Piret, E. Galopin, Y. Coffinier, R. Boukherroub, D. Legrand and C. Slomianny, Soft Matter, 2011, 7, 8642 DOI: 10.1039/C1SM05838J

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