Issue 7, 2012

A quartz nanopillar hemocytometer for high-yield separation and counting of CD4+ T lymphocytes

Abstract

We report the development of a novel quartz nanopillar (QNP) array cell separation system capable of selectively capturing and isolating a single cell population including primary CD4+ T lymphocytes from the whole pool of splenocytes. Integrated with a photolithographically patterned hemocytometer structure, the streptavidin (STR)-functionalized-QNP (STR-QNP) arrays allow for direct quantitation of captured cells using high content imaging. This technology exhibits an excellent separation yield (efficiency) of ∼95.3 ± 1.1% for the CD4+ T lymphocytes from the mouse splenocyte suspensions and good linear response for quantitating captured CD4+ T-lymphoblasts, which is comparable to flow cytometry and outperforms any non-nanostructured surface capture techniques, i.e. cell panning. This nanopillar hemocytometer represents a simple, yet efficient cell capture and counting technology and may find immediate applications for diagnosis and immune monitoring in the point-of-care setting.

Graphical abstract: A quartz nanopillar hemocytometer for high-yield separation and counting of CD4+ T lymphocytes

Supplementary files

Article information

Article type
Paper
Submitted
18 Sep 2011
Accepted
22 Nov 2011
First published
04 Jan 2012

Nanoscale, 2012,4, 2500-2507

A quartz nanopillar hemocytometer for high-yield separation and counting of CD4+ T lymphocytes

D. Kim, J. Seol, Y. Wu, S. Ji, G. Kim, J. Hyung, S. Lee, H. Lim, R. Fan and S. Lee, Nanoscale, 2012, 4, 2500 DOI: 10.1039/C2NR11338D

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