Original contributionAn immunohistochemical study of metaplastic spindle cell carcinoma, phyllodes tumor and fibromatosis of the breast
Section snippets
Materials and methods
We collected 18 MSCs (16 biphasic, 2 uniphasic), 26 PTs (18 benign, 5 borderline, 3 malignant), and 8 fibromatoses from the files of Nottingham City Hospital. All tumors had been resected at this hospital between 1987 and 1999. We reviewed the hematoxylin and eosin (H&E) slides. The PTs were graded according to the criteria of Moffat et al.27
We performed immunohistochemistry on one representative block from each case for Cam5.2 (Becton Dickinson, Franklin Lakes, NJ, 1:2), AE1/AE3 (Dako,
Age and sex of patients
All patients were female. The age range for the MSC patients was 27–82 years, and the mean, 57 years (standard deviation [SD], ± 16.3); for the PT patients, it was 20–92 years, and the mean, 52 years (± SD 12.9); and for the fibromatoses, it was 20–64 years, and the mean, 44 years (± SD 19.4).
Macroscopy and microscopic review
Gross pathology was detailed from the pathology report. The macroscopic size for the MSCs ranged from 10 to 80 mm in maximum extent. Where circumscription was documented, 9 were described as well defined,
Discussion
We reviewed all of the spindle cell tumors of breast that were recorded in our practice over a 12-year period. We carried out a detailed immunohistochemical analysis of the tumors by using antibodies against a range of CKs and mesenchymal markers. Our aim was to try to better understand the histogenesis of these tumors and to identify potential antibodies that might aid the histopathologist in his or her diagnosis.
The mesenchymal elements of the MSCs showed heterogeneous immunohistochemical
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