Alimentary TractProbiotic bacteria enhance murine and human intestinal epithelial barrier function☆,☆☆
Section snippets
Animals
Homozygous IL-10 gene-deficient mice, generated on a 129 Sv/Ev background, and normal 129 Sv/Ev controls were housed behind a barrier under specific pathogen-free conditions. All provisions for the facility were sterilized using an autoclave. Nonautoclavable supplies were sprayed with disinfectant and introduced into the facility through a high-efficiency particulate air (HEPA)-filtered air-lock. The facility's air was HEPA-filtered. The mice were housed in micro isolator cages covered with
Colonic bacteria
To confirm that the oral administration of the VSL#3 compound resulted in viable organisms reaching the colon, total and adherent levels of Lactobacillus spp. and Bifidobacteria spp. were measured in control and IL-10 gene-deficient mice after 4 weeks of treatment. There was no difference between controls and IL-10 gene-deficient mice in the total amount of Lactobacillus and Bifidobacteria (Figure 1A).
Discussion
In the present study, we have shown that treatment of IL-10 gene-deficient mice with the probiotic compound, VSL#3, results in a normalization of colonic physiologic function and barrier integrity in conjunction with a reduction in mucosal levels of proinflammatory cytokines and a significant improvement in histologic disease. Furthermore, in vitro studies show that epithelial barrier function can be modulated by exposure to proteinaceous soluble factor(s) secreted by the probiotic bacteria
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Address requests for reprints to: Karen L. Madsen, Ph.D., University of Alberta, 536 Newton Building, Edmonton, Alberta, Canada T6G 2C2. e-mail: [email protected]; fax: (780) 407-3744.
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Supported by grants from Alberta Heritage Foundation for Medical Research, Crohn's and Colitis Foundation of Canada, and Gastrointestinal Liver Disease Research Group.