Basic-alimentary tractTherapeutic effects of rectal administration of basic fibroblast growth factor on experimental murine colitis
Section snippets
Animals
Female C57BL/6 mice (8–10 weeks old; Japan SLC, Inc, Shizuoka, Japan) and female SJL/J mice (10–11 weeks old; Charles River Japan, Inc, Kanagawa, Japan) were used for the experiments. They were fed with standard laboratory chow and tap water ad libitum. All mice were housed in specific pathogen-free conditions in the animal facility of Kyoto University. The studies were approved by the animal protection committee of our institution.
Induction of colitis
To induce colitis, C57BL/6 mice were given 3% DSS (mol wt,
Body weight change
In the nontreated mice with DSS-induced colitis, body weight gradually decreased and did not recover at the end of the experiment. On the other hand, the body weight in mice with DSS-induced colitis treated with 1.0 or 5.0 mg/kg of hrbFGF significantly recovered in a dose-dependent manner compared with the nontreated mice with DSS-induced colitis (Figure 2).
Colonic length
The colonic length in the nontreated mice with DSS-induced colitis was significantly shorter than in normal mice. The colonic length in
Discussion
For the past 10 years, several growth factors, such as EGF, FGF, insulin-like growth factor, and hepatocyte growth factor, have been tested as potent therapeutic agents in a variety of experimental models of human IBD.30, 31, 32, 33, 34, 35, 36 Among them, a great deal of attention has been paid to the FGF family, especially KGF-1 and KGF-2, because several clinical and experimental studies showed that they are also effective for the treatment of epithelial damage in other tissues, such as skin
References (66)
Inflammatory bowel diseaseetiology and pathogenesis
Gastroenterology
(1998)- et al.
Regulation of the mucosal epithelial barrier
Baillieres Clin Gastroenterol
(1996) - et al.
Adv Cancer Res
(1992) - et al.
Effect of bFGF on the inhibition of contraction caused by bacteria
J Surg Res
(1991) - et al.
Fibroblast growth factors modulate intestinal epithelial cell growth and migration
Gastroenterology
(1994) - et al.
A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice
Gastroenterology
(1990) - et al.
Enhanced survival and mucosal repair after dextran sodium sulfate-induced colitis in transgenic mice that overexpress growth hormone
Gastroenterology
(2001) - et al.
Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro
Anal Biochem
(1999) - et al.
Protective effect of epidermal growth factor in an experimental model of colitis in rats
Gastroenterology
(1994) - et al.
A novel human fibroblast growth factor treats experimental intestinal inflammation
Gastroenterology
(2002)
Keratinocyte growth factor-2 accelerates wound healing in incisional wounds
J Surg Res
Immunohistochemical analysis of cell kinetic parameters in colonic adenocarcinomas, adenomas, and normal mucosa
Hum Pathol
Signaling through FGFR3 is neccesary for expansion of the epithelial stem cell compartment during normal intestinal development
Gastroenterology
Transforming growth factor beta regulation of migration in wounded rat intestinal epithelial monolayers
Gastroenterology
Trefoil peptide protection of intestinal epithelial barrier functioncooperative interaction with mucin glycoprotein
Gastroenterology
Prostaglandins prevent decreased epithelial cell proliferation associated with dextran sodium sulfate injury in mice
Gastroenterology
In vitro cytokine release by activated murine peritoneal macrophagesrole of prostaglandins in the differential regulation of tumor necrosis factor alpha, interleukin 1, and interleukin 6
Cytokine
Basic fibroblast growth factor induces cyclooxygenase-2 expression in endothelial cells derived from bone
Biochem Biophys Res Commun
Intestinal microflora in human and experimental inflammation bowel disease
Curr Opin Gastroenterol
Mechanisms and modulation of intestinal epithelial repair
Inflamm Bowel Dis
Peptide growth factors in the intestine
Eur J Gastroenterol Hepatol
Repifermin (keratinocyte growth factor-2) for the treatment of active ulcerative colitisa randomized, double-blind, placebo-controlled, dose-escalation trial
Aliment Pharmacol Ther
Epidermal growth factor enemas with oral mesalamine for mild-to-moderate left-sided ulcerative colitis or proctitis
N Engl J Med
Growth control and morphogenesis in the development and pathology of arteries
J Cardiovasc Pharmacol
Salvage of infarcted myocardium by angiogenic action of basic fibroblast growth factor
Science
Basic fibroblast growth factor improves myocardial function in chronically ischemic porcine hearts
J Clin Invest
Biological roles of fibroblast growth factor-2
Endocr Rev
[Effect of recombinant human basic fibroblast growth factor (rh-bFGF) on wound healing of the corneal epithelium]
Ophthalmologe
Basic fibroblast growth factor stimulates corneal endothelial cell growth and endothelial wound healing of human corneas
Invest Ophthalmol Vis Sci
Accelerated healing of laser-injured rabbit retina by basic fibroblast growth factor
Invest Ophthalmol Vis Sci
Dose responsive effects of PDGF-BB, PDGF-AA, EGF, and bFGF on granulation tissue in a guinea pig partial thickness skin excision model
Growth Factors
PDGF and FGF reverse the healing impairment in protein-malnourished diabetic mice
Surgery
Basic fibroblast growth factor protects against hypoxia-ischemia and NMDA neurotoxicity in neonatal rats
J Cereb Blood Flow Metab
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2021, BurnsCitation Excerpt :It regulates a variety of biological functions through complex signal transduction systems, including proliferation, morphogenesis and inhibition of apoptosis during development [14]. A previous study suggested that rectal administration of hrbFGF ameliorated colitis [15]. We investigated the protective effect of exogenous bFGF treatment on intestinal mucosal integrity.
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2015, ImmunityCitation Excerpt :In addition to IL-17, the mitogenic growth factor FGF2 (also named bFGF) is also increased in IBD (Bousvaros et al., 1997; Fujino et al., 2003; Thörn et al., 2000). Although administration of recombinant FGF2 alleviates DSS-induced colitis pathology (Matsuura et al., 2005), it is not clear how FGF2 functions during the colitis pathogenesis. FGF2 associates with its homodimer receptor FGFR1.
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2014, Journal of Functional FoodsCitation Excerpt :COX-2 expression was also increased by DSS in colon tissue as compared with that in the control group, and COX-2 expression was decreased by YPEE (Fig. 3). The importance of COX-2 in ulcerative colitis has been shown in earlier studies indicating that a selective COX-2 inhibitor has beneficial health effects in both 2,4,6-trinitrobenzenesulfonic acid (TNBS)- and DSS-induced colitis in animal models, partly by reducing IL-1β levels (Algieri et al., 2014; Davaatseren et al., 2013; Matsuura et al., 2005). Recent study has been reported that hesperidin and quercetin ameliorate DSS-induced colitis (Mizuki, Soichi, & Takuya, 2013).
Supported in part by the Establishment of International COE for Integration of Transplantation Therapy and Regenerative Medicine (COE program of the Ministry of Education, Culture, Sports, Science, and Technology, Japan); Grant-in-Aid for Scientific Research (C) from the Ministry of Culture and Science in Japan (16560645); and Health and Labour Science Research Grants from the Japanese Ministry of Health, Labour and Welfare, and Research on Measures for Intractable Disease (Inflammatory Bowel Disease).