Basic-alimentary tractMast Cells Are Critical Mediators of Vaccine-Induced Helicobacter Clearance in the Mouse Model
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Mice
WB/ReJ-KitW/+ and C57BL/6J-KitWv/+ mice were purchased from the Jackson Laboratory (Bar Harbor, ME) and mated in our SPF animal facility. We used either male or female 6- to 8-week-old W/Wv and WBB6F1 +/+ mice. Female Balb/c mice (6 to 8 weeks old) were purchased from Harlan, Horst, The Netherlands. This study was approved by the State of Vaud Veterinary Office (authorization No. 836.6).
Immunization, In Vivo CD4+ Cells Depletion, and Mastocytosis Induction
Mice were immunized intranasally 4 times at 1-week intervals with 30 μg recombinant H pylori urease (kindly
Kinetics Studies of Vaccination-Mediated Clearance of Helicobacter
We first studied the kinetics of H felis clearance in wild-type mice vaccinated intranasally with urease + CT. At day 4 postchallenge, infection, as defined by positive urease tests on gastric mucosa samples, was detected in 4 out of 5 urease + CT-vaccinated mice (Figure 1A). At day 5 postchallenge, only 2 out of 10 vaccinated mice showed positive urease tests, whereas 10 out of 10 control mice vaccinated with CT alone were positive. Gastric histologic analysis confirmed the presence of H felis
Discussion
Vaccination against H pylori is an important goal in the prevention of gastroduodenal diseases, including ulcers and gastric malignancies. Despite the proven ability of vaccination to prevent or reduce Helicobacter infection in animal models, the precise mechanisms of protection have remained obscure. Mucosal and systemic immunizations with Helicobacter extracts or purified recombinant proteins generate antigen-specific serum and salivary and intestinal antibody responses as well as cellular
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Selected recent advances in understanding the role of human mast cells in health and disease
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2016, American Journal of PathologyCitation Excerpt :Hence, the latter findings provide further support for a role of MCs in regulating adaptive immune responses against bacteria.29 Consistent with this, Shelburne et al25 showed that MCs promoted the antibody response to E. coli, and it has been demonstrated that MCs play a critical role in anti-Helicobacter vaccination.39 Several studies have indicated that MCs can act directly on bacteria, although it should be recognized that there is only limited in vivo evidence to support this notion.
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Supported by the Swiss National Foundation (grant 3100-068243 to P.M.).