Elsevier

Metabolism

Volume 50, Issue 5, May 2001, Pages 553-557
Metabolism

Increased protein kinase C theta in skeletal muscle of diabetic patients

https://doi.org/10.1053/meta.2001.22512Get rights and content

Abstract

In this study we have investigated whether protein kinase C (PKC) protein and activity are increased in skeletal muscle of human diabetic patients. The protein content of different PKC isoforms (β, Θ, ϵ, δ, μ, and ζ) in the particulate fraction was measured, using Western analysis, in human rectus abdominus skeletal muscle from obese (hyperinsulinemic, normoglycemic) and obese diabetic (hyperinsulinemic, hyperglycemic) subjects. PKC Θ protein content was significantly higher in the particulate fraction of muscle from diabetic patients compared with the nondiabetic controls. PKC Θ was immunoprecipitated and its activity was measured in muscle from diabetic and nondiabetic controls. There was a significant increase in PKC Θ activity in muscle from diabetic patients compared with muscle from nondiabetic controls. Therefore, both PKC Θ protein content and activity were significantly increased in the particulate fraction in muscle from diabetic patients, suggesting the involvement of this isoform in diabetes. Most of the PKC Θ protein was found in the cytosol. There was no change in cytosolic PKC Θ protein content in muscle from diabetic patients compared with muscle from nondiabetic controls. Thus, the increase in particulate-associated PKC Θ was likely due to translocation and activation rather than an increase in protein mass.

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Supported by Grant No. DK46121 from the National Institutes of Health.

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