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DOI: 10.1055/s-0028-1093851
© Georg Thieme Verlag KG Stuttgart · New York
Group Experiments on the Radioimmunological Insulin Determination[*]
Publication History
Publication Date:
08 January 2009 (online)
Abstract
By means of two group experiments, performed in seven laboratories and using six different modifications in the insulin radioimmunoassay technique, it is shown that the use of standards diluted in human serum of low basal insulin concentration, instead of buffer, improves appreciably the interlaboratory precision. The causes lies perhaps in some serum factor, which could disturb some modifications of the radioimmunoassay more than others. There was no improvement of precision when the same insulin standard was used in all laboratories. Standard curves made up in different human fasting sera have a scatter only within the range of pipetting and counting error.
Key words
Group Experiment - Insulin RIA - Data Processing - Quality Control - Insulin Recovery
1 Supported by the Deutsche Forschungsgemeinschaft (SFB 51, München, and SFB 87, Ulm).
1 Supported by the Deutsche Forschungsgemeinschaft (SFB 51, München, and SFB 87, Ulm).