Horm Metab Res 1974; 6(4): 293-296
DOI: 10.1055/s-0028-1093851
Originals

© Georg Thieme Verlag KG Stuttgart · New York

Group Experiments on the Radioimmunological Insulin Determination[*]

I.  Marschner1 , P.  Bottermann1 , F.  Erhardt1 , R.  Linke2 , G.  Löffler1 , V.  Maier3 , P.  Schwandt1 , W.  Vogt1 , P. C. Scriba1
  • 1Sonderforschungsbereich 51 Muenchen, Germany
  • 2Sonderforschungsbereich Tutzing, Germany
  • 3Sonderforschungsbereich 87 Ulm, Germany
Further Information

Publication History

Publication Date:
08 January 2009 (online)

Abstract

By means of two group experiments, performed in seven laboratories and using six different modifications in the insulin radioimmunoassay technique, it is shown that the use of standards diluted in human serum of low basal insulin concentration, instead of buffer, improves appreciably the interlaboratory precision. The causes lies perhaps in some serum factor, which could disturb some modifications of the radioimmunoassay more than others. There was no improvement of precision when the same insulin standard was used in all laboratories. Standard curves made up in different human fasting sera have a scatter only within the range of pipetting and counting error.

1 Supported by the Deutsche Forschungsgemeinschaft (SFB 51, München, and SFB 87, Ulm).

1 Supported by the Deutsche Forschungsgemeinschaft (SFB 51, München, and SFB 87, Ulm).

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