Planta Med 2002; 68(2): 119-122
DOI: 10.1055/s-2002-20240
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Inhibitory Effect of Tumor Cell Proliferation and Induction of G2/M Cell Cycle Arrest by Panaxytriol

Ji Yeon Kim1 , Ki-Won Lee1 , Sun-Ho Kim1 , Jae Joon Wee2 , Young-Suk Kim3 , Hyong Joo Lee1
  • 1Department of Food Science and Technology and School of Agricultural Biotechnology, Seoul National University, Suwon, Korea
  • 2Korea Ginseng and Tobacco Research Institute, Taejon, Korea
  • 3Department of Food and Nutrition, Ewha Womans University, Seoul, Korea
Further Information

Publication History

February 1, 2001

April 28, 2001

Publication Date:
22 February 2002 (online)

Abstract

Panaxytriol, a polyacetylenic compound, isolated from red ginseng (Panax ginseng C.A. Meyer), was studied to determine its effects on the growth and cell cycle of tumor cell lines. The compound showed both significant cytotoxicity and inhibition of DNA syntheses in various tumor cells tested. For P388D1, a mouse lymphoma cell line, IC50 values for cytotoxicity and inhibition of DNA synthesis were 3.1 and 0.7 μg/ml, respectively. The cytotoxic effect of panaxytriol was both time- and dose-dependent. It also induced the cell cycle arrest of P388D1 at the G2/M phase, which was measured through flow cytometry. Particularly, the proportion of cells in the G2/M phase of the cell cycle increased from 9 % to 26 and 48 %, respectively, after 24 and 36 h exposure to panaxytriol at 5 μg/ml. There were corresponding decreases in the proportion of cells at the G0/G1 phase. The S phase also decreased during the 36-h treatment.

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Prof. Dr. Hyong Joo Lee

Department of Food Science and Technology and School of Agricultural Biotechnology

Seoul National University

Suwon, 441-744

Korea

Email: leehyjo@snu.ac.kr

Fax: +82-31 293 4789

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