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Planta Med 2002; 68(11): 966-970
DOI: 10.1055/s-2002-35667
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Tubuloside B from Cistanche salsa Rescues the PC12 Neuronal Cells from 1-Methyl-4-phenylpyridinium Ion-Induced Apoptosis and Oxidative Stress

Guoqing Sheng1 , Xiaoping Pu1 , Li Lei2 , Pengfei Tu2 , Changling Li1
  • 1Department of Molecular and Cellular Pharmaoclogy, School of Pharmaceutical Sciences, Peking University, Beijing, China
  • 2Department of Natural Product, School of Pharmaceutical Sciences, Peking University, Beijing, China
Further Information

Publication History

Received: March 18, 2002

Accepted: June 29, 2002

Publication Date:
26 November 2002 (online)

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Abstract

The neuroprotective effects of tubuloside B, one of the phenylethanoids isolated from the Chinese herbal medicine Cistanche salsa, on 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis and oxidative stress in PC12 neuronal cells were investigated. PC12 cells treated with MPP+ underwent apoptotic death as determined by MTT assay, flow cytometry and DNA agarose gel electrophoresis; intracellular accumulation of reactive oxygen species (ROS) was measured by DCFH-DA staining with laser scanning confocal microscopy (LSCM). Simultaneous treatment with tubuloside B markedly attenuated MPP+-induced cytotoxicity, DNA fragmentation, and intracellular accumulation of ROS. These results strongly indicate that tubuloside B prevents MPP+-induced apoptosis and oxidative stress. Tubuloside B may be applied as an antiparkinsonian agent.

Key words

Cistanche salsa - Orobanchaceae - tubuloside B - apoptosis - reactive oxygen species - oxidative stress - Parkinson's disease

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Prof. Changling Li,
Prof. Xiaoping Pu

Department of Molecular and Cellular Pharmacology

School of Pharmaceutical Sciences

Peking University

Beijing 100083

China

Email: lichangling@bjmu.edu.cn

Fax: +86-10-62092648

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