Subscribe to RSS
DOI: 10.1055/s-2006-957570
© Georg Thieme Verlag Stuttgart · New York
High Performance Liquid Chromatography in Phytochemical Analysis of Eurycoma longifolia
Publication History
1998
1998
Publication Date:
04 January 2007 (online)
Abstract
An analytical method using HPLC with UV detection was developed to investigate the quassinoid content of Eurycoma longifolia Jack (Simaroubaceae) collected from various sources. Eurycomanone (1), longilactone (2), 14,15β-dihydroxyklaineanone (3), 15β-acetyl-14-hydroxyklaineanone (4), 6α-hydroxyeurycomalactone (5), and eurycomalactone (7) were isolated as reference standards and together with the synthesized 1β,12α,15β-triacetyleurycomanone (6, internal standard), were identified by NMR, MS, UV and IR spectroscopies. Their coefficient of variation values for 0.50-35 µg ml-1 concentrations of quassinoids and their retention times measured within- and between-day were small. The recoveries of the spiked quassinoids in E. longifolia samples and their detection limits at 8.5 times signal to noise ratio were 99.75-109.13% and 0.01 µg ml-1, respectively. From the root samples analysed, 1 had the highest concentration, being about 16.8-39.6 fold higher than the other quassinoids 2, 3, 5, 7 but 145.3 fold higher than 4 which showed the lowest concentration.
Key words
Eurycoma longifolia - Simaroubaceae - quassinoids analysis - HPLC-UV