Journal of Biological Chemistry
Volume 270, Issue 44, 3 November 1995, Pages 26104-26108
Journal home page for Journal of Biological Chemistry

Nucleic Acids, Protein Synthesis, and Molecular Genetics
A Chinese Hamster Mutant Cell Line with a Defect in the Integral Membrane Protein CII-3 of Complex II of the Mitochondrial Electron Transport Chain (∗)

https://doi.org/10.1074/jbc.270.44.26104Get rights and content
Under a Creative Commons license
open access

In this study, a respiration-deficient Chinese hamster cell line with a defect in succinate dehydrogenase activity is shown to result from a single base change in a codon in the coding sequence for the membrane anchor protein CII-3 (also referred to as QPs-1). A premature translation stop results in the truncation of 33 amino acids from the C terminus. Bovine cDNA encoding this peptide complements the mutation. There is about 82% identity between these two mammalian proteins. The gene for CII-3 was mapped on human chromosome 1, and because it is also found on minichromosomes characterized by our laboratory, we can localize it on the short arm within 1-2 megabases from the centromere.

Cited by (0)

The work was supported by National Public Health Service Grant GM33752 (to I. E. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank(TM)/EMBL Data Bank with accession number(s) U31241.

§

Current address: Faculty of Biology, Vrije Universiteit, Amsterdam, The Netherlands.

Current address: University of Lund, Lund, Sweden.