Journal of Biological Chemistry
Cell Biology and MetabolismA Population of Rat Liver Lysosomes Responsible for the Selective Uptake and Degradation of Cytosolic Proteins*
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This work was supported by a post-doctoral grant Fundacion Ramon Areces (Spain) (to A. M. C.), by National Institutes of Health Grant AG06116 (to J. F. D.), and by Dirección General de Investigación Científica y Técnica del Ministerio de Educación y Ciencia Grant PB94-1281 and Fondo de Investigación Sanitaria de la Seguridad Social 93/0498 (to E. K.). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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The abbreviations used are:
- RNase A
ribonuclease A
- RNase S-peptide
residues 1-20 of RNase A
- GAPDH
glyceraldehyde-3-phosphate dehydrogenase
- hsc73
heat shock cognate protein of 73 kDa
- hsp70
heat shock protein of 70 kDa
- FITC
fluorescein isothiocyanate
- lgp120
lysosomal glycoprotein of 120 kDa
- GST
glutathione transferase
- MOPS
3-(N-morpholino) propanesulfonic acid
- PAGE
polyacrylamide gel electrophoresis.