Characterization of a Novel Type of Serine/Threonine Kinase That Specifically Phosphorylates the Human Goodpasture Antigen

doi:10.1074/jbc.274.18.12642

Journal of Biological Chemistry

Volume 274, Issue 18, 30 April 1999, Pages 12642-12649

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Goodpasture disease is an autoimmune disorder that occurs naturally only in humans. Also exclusive to humans is the phosphorylation process that targets the unique N-terminal region of the Goodpasture antigen. Here we report the molecular cloning of GPBP (Goodpasture antigen-bindingprotein), a previously unknown 624-residue polypeptide. Although the predicted sequence does not meet the conventional structural requirements for a protein kinase, its recombinant counterpart specifically binds to and phosphorylates the exclusive N-terminal region of the human Goodpasture antigen in vitro. This novel kinase is widely expressed in human tissues but shows preferential expression in the histological structures that are targets of common autoimmune responses. The work presented in this report highlights a novel gene to be explored in human autoimmunity.

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This work was supported in part by Grants SAL91/0513, SAF94/1051, and SAF97/0065 from the Plan Nacional I+D of the Comisión Interministerial de Ciencia Tecnologı́a, Grant 93/0343 from Fondo de Investigaciones Sanitarias, and Grants GV-3166/95 and GV-C-VS-21-118-96 from la Direcció General d'Ensenyaments Universitaris i Investigació (Comunitat Valenciana, Spain) (to J. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number(s) AF136450.

^§: Supported by a postdoctoral fellowship sponsored by Fundación Valenciana de Investigaciones Biomédicas, by Exploraciones Radiológicas Especiales S.A., and by Bancaixa.

^¶: Recipient of a fellowship from the Consellerı́a de Educació i Ciència de la Comunitat Valenciana.

Journal of Biological Chemistry

NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICSCharacterization of a Novel Type of Serine/Threonine Kinase That Specifically Phosphorylates the Human Goodpasture Antigen*

NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS
Characterization of a Novel Type of Serine/Threonine Kinase That Specifically Phosphorylates the Human Goodpasture Antigen*