Journal of Biological Chemistry
MECHANISMS OF SIGNAL TRANSDUCTIONErk Is Essential for Growth, Differentiation, Integrin Expression, and Cell Function in Human Osteoblastic Cells*
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Published, JBC Papers in Press, January 29, 2001, DOI 10.1074/jbc.M010021200
- FGF
fibroblast growth factor
- TGF
transforming growth factor
- Erk
extracellular signal-regulated kinase
- Erk1DN
dominant negative Erk1
- HIFBS
heat-inactivated fetal bovine serum
- HOB cell
human osteoblastic cell
- MAPK
mitogen-activated protein kinase
- α-MEM
α-minimum Eagle's medium
- PBS
phosphate-buffered saline
- PT
phosphate-buffered saline with 0.1% Triton X-100
- DTT
dithiothreitol
- PMSF
phenylmethylsulfonyl fluoride
- PAGE
polyacrylamide gel electrophoresis
- CLS
Coffin-Lowry syndrome
- MOPS
4-morpholinepropanesulfonic acid
- BMP
bone morphogenetic protein
- BSA
bovine serum albumin
- JNK
c-Jun N-terminal kinase
- FAK
focal adhesion kinase
- EMSA
electrophoretic mobility shift assay
The abbreviations used are:
- *
This work was supported by National Institutes of Health Grants AR32087 and AR07033.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
This paper is dedicated to the memory of Dr. Louis V. Avioli.
- †
Deceased.
- ‖
To whom all correspondence should be addressed: Div. of Bone and Mineral Diseases, Barnes-Jewish Hospital of St. Louis, Washington University School of Medicine, 216 S. Kingshighway Blvd., St. Louis, MO 63110. Tel.: 314-454-8406; Fax: 314-454-5047;