GENES: STRUCTURE AND REGULATION
Rvb1p and Rvb2p Are Essential Components of a Chromatin Remodeling Complex That Regulates Transcription of over 5% of Yeast Genes*

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Eukaryotic Rvb1p and Rvb2p are two highly conserved proteins related to the helicase subset of the AAA+ family of ATPases. Conditional mutants in both genes show rapid changes in the transcription of over 5% of yeast genes, with a similar number of genes being repressed and activated. Both Rvb1p and Rvb2p are required for maintaining the induced state of many inducible promoters. ATP binding and hydrolysis by Rvb1p and Rvb2p is individually essentialin vivo, and the two proteins are associated with each other in a high molecular weight complex that shows ATP-dependent chromatin remodeling activity in vitro. Our findings show that Rvb1p and Rvb2p are essential components of a chromatin remodeling complex and determine genes regulated by the complex.

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Published, JBC Papers in Press, February 5, 2001, DOI 10.1074/jbc.M011523200

*

This work was supported in part by grants from the Swiss National Fund for Science (to Z. O. J.) and National Institutes of Health Grant CA60499 (to A. D.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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Supported by Postdoctoral Fellowship DAMD17-00-1-0166 from the United States Army Medical Research Acquisition Activity.

Supported by a predoctoral fellowship from Howard Hughes Medical Institutes.