Journal of Biological Chemistry
GLYCOBIOLOGY AND EXTRACELLULAR MATRICESSequence Analysis of Heparan Sulfate Epitopes with Graded Affinities for Fibroblast Growth Factors 1 and 2*
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Published, JBC Papers in Press, June 13, 2001, DOI 10.1074/jbc.M102628200
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Unit 1 is invariably GlcUA in all oligosaccharides generated by theN-deacetylation/deamination process, because the -GlcNAc-GlcUA- sequence (cleaved in this process) is not a substrate for the C-5 epimerase that converts GlcUA to IdoUA during heparin/HS biosynthesis (44).
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Dependent on the charge density of the saccharide analyzed, removal of terminal IdoUA monosaccharide or GlcUA-GlcNSO3 disaccharide units variously affected the elution position in anion-exchange chromatograms. Generally, parental fragments eluted before 50 min were shifted to the left, and those emerging between 50 and 70 min were only slightly affected, whereas components appearing after 70 min were shifted to the right.
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This work was supported by European Comission Grants QLK-CT-1999.00536 (Program “Biologically Active Novel Glycosaminoglycans”) and BIO4-CT98-0538 (Program “Heparan Sulfate Sequencing Demonstration”), Medical Research Council Grant K96-03P,K99-03X,2309, Swedish Cancer Society Grant 3919-B97, and by funds from the Finnish Cancer Union, the Sigrid Juselius Foundation, and Polysackaridforskning AB.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.