Journal of Biological Chemistry
Volume 285, Issue 34, 20 August 2010, Pages 26223-26232
Journal home page for Journal of Biological Chemistry

Immunology
A Role for the Human Nucleotide-binding Domain, Leucine-rich Repeat-containing Family Member NLRC5 in Antiviral Responses*

https://doi.org/10.1074/jbc.M110.109736Get rights and content
Under a Creative Commons license
open access

Proteins of the nucleotide-binding domain, leucine-rich repeat (NLR)-containing family recently gained attention as important components of the innate immune system. Although over 20 of these proteins are present in humans, only a few members including the cytosolic pattern recognition receptors NOD1, NOD2, and NLRP3 have been analyzed extensively. These NLRs were shown to be pivotal for mounting innate immune response toward microbial invasion. Here we report on the characterization of human NLRC5 and provide evidence that this NLR has a function in innate immune responses. We found that NLRC5 is a cytosolic protein expressed predominantly in hematopoetic cells. NLRC5 mRNA and protein expression was inducible by the double-stranded RNA analog poly(I·C) and Sendai virus. Overexpression of NLRC5 failed to trigger inflammatory responses such as the NF-κB or interferon pathways in HEK293T cells. However, knockdown of endogenous NLRC5 reduced Sendai virus- and poly(I·C)-mediated type I interferon pathway-dependent responses in THP-1 cells and human primary dermal fibroblasts. Taken together, this defines a function for NLRC5 in anti-viral innate immune responses.

Innate Immunity
Interferon
Pattern Recognition Receptor
Signal Transduction
Viral Immunology
CLR16.1
Nod27
Sendai Virus

Cited by (0)

*

This work was supported by Deutsche Forschungsgemeinschaft Grants SFB670-N01 (to T. A. K.) and SFB670-TP8 (to M. H. and H. B.) and European Commission Grant MEXT-CT-2006-033534 (to R. S. and A. N.).

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1–S3.