Journal of Biological Chemistry
Volume 287, Issue 34, 17 August 2012, Pages 28632-28645
Journal home page for Journal of Biological Chemistry

Neurobiology
Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes*

https://doi.org/10.1074/jbc.M111.336644Get rights and content
Under a Creative Commons license
open access

The AMPA receptor (AMPA-R) is a major excitatory neurotransmitter receptor in the brain. Identifying and characterizing the neuronal proteins interacting with AMPA-Rs have provided important information about the molecular mechanisms underlying synaptic transmission and plasticity. In this study, to identify more AMPA-R interactors in vivo, we performed proteomic analyses of AMPA-R complexes from the brain. AMPA-R complexes were isolated from the brain through various combinations of biochemical techniques for solubilization, enrichment, and immunoprecipitation. Mass spectrometry analyses of these isolated complexes identified several novel components of the AMPA-R complexes as well as some previously identified components. The identification of these novel components helps to further define the complex mechanisms involved in the regulation of AMPA receptor function and synaptic plasticity.

Glutamate Receptors Ionotropic (AMPA, NMDA)
Neurochemistry
Neuroscience
Neurotransmitter Receptors
Protein Complexes
Protein Purification
Protein-Protein Interactions
Proteomics
Synaptic Plasticity
Synaptic Transmission

Cited by (0)

*

This work was supported, in whole or in part, by National Institutes of Health Grants R01NS036715 (to R. L. H.) and N01-HV-28180 (to Y. G.).

This article contains supplemental Figs. 1–7.