Journal of Biological Chemistry
Volume 278, Issue 13, 28 March 2003, Pages 11393-11401
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MOLECULAR BASIS OF CELL AND DEVELOPMENTAL BIOLOGY
Association of the Kinesin Motor KIF1A with the Multimodular Protein Liprin-α*

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Liprin-α/SYD-2 is a multimodular scaffolding protein important for presynaptic differentiation and postsynaptic targeting of α-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid glutamate receptors. However, the molecular mechanisms underlying these functions remain largely unknown. Here we report that liprin-α interacts with the neuron-specific kinesin motor KIF1A. KIF1A colocalizes with liprin-α in various subcellular regions of neurons. KIF1A coaccumulates with liprin-α in ligated sciatic nerves. KIF1A cofractionates and coimmunopreciptates with liprin-α and various liprin-α-associated membrane, signaling, and scaffolding proteins including α-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid receptors, GRIP/ABP, RIM, GIT1, and βPIX. These results suggest that liprin-α functions as a KIF1A receptor, linking KIF1A to various liprin-α-associated proteins for their transport in neurons.

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*

This work was supported by grants from the Korean Ministry of Science and Technology, the Korea Research Foundation, and the Korea Science and Engineering Foundation (to E. K.) and National Institutes of Health Grant NS-35527 (to R. J. W.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Amgen Inc., Thousand Oaks, CA 91320.

Present address: ImmunoGen, Inc., 128 Sidney St., Cambridge, MA 02139.

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Associate investigator of the Howard Hughes Medical Institute.