Journal of Biological Chemistry
Volume 282, Issue 46, 16 November 2007, Pages 33305-33312
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Mechanisms of Signal Transduction
Soluble Aβ Inhibits Specific Signal Transduction Cascades Common to the Insulin Receptor Pathway*

https://doi.org/10.1074/jbc.M610390200Get rights and content
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Numerous studies have now shown that the amyloid β-protein (Aβ), the principal component of cerebral plaques in Alzheimer disease, rapidly and potently inhibits certain forms of synaptic plasticity. The amyloid (or Aβ) hypothesis proposes that the continuous disruption of normal synaptic physiology by Aβ contributes to the development of Alzheimer disease. However, there is little consensus about how Aβ mediates this inhibition at the molecular level. Using mouse primary hippocampal neurons, we observed that a brief treatment with cell-derived, soluble, human Aβ disrupted the activation of three kinases (Erk/MAPK, CaMKII, and the phosphatidylinositol 3-kinase-activated protein Akt/protein kinase B) that are required for long term potentiation, whereas two other kinases (protein kinase A and protein kinase C) were stimulated normally. An antagonist of the insulin receptor family of tyrosine kinases was found to mimic the pattern of Aβ-mediated kinase inhibition. We then found that soluble Aβ binds to the insulin receptor and interferes with its insulin-induced autophosphorylation. Taken together, these data demonstrate that physiologically relevant levels of naturally secreted Aβ interfere with insulin receptor function in hippocampal neurons and prevent the rapid activation of specific kinases required for long term potentiation.

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*

This work was supported by National Institutes of Health Grants AG027443 (to D. J. S.) and T32 NS07484-04 and Massachusetts Alzheimerʼns Disease Research Center (to M. T.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1 and S2.