YadA, the multifaceted Yersinia adhesin

doi:10.1078/1438-4221-00119

International Journal of Medical Microbiology

Volume 291, Issue 3, 2001, Pages 209-218

https://doi.org/10.1078/1438-4221-00119 Get rights and content

Abstract

The adhesion protein YadA is encoded by the yadAgene located in the 70-kb virulence plasmid of Yersinia (pYV) that is common to the pathogenic Yersinia species (Y. pestis, Y. pseudotuberculosis and Y. enterocolitica). YadA is a virulence factor of Y. enterocolitica, however, YadA seems to be dispensable for the virulence of Y. pseudotuberculosis, and in wild-type Y. pestis the yadA gene has a frameshift mutation silencing the gene. Expression of the Y. pseudotuberculosisYadA in Y. pestis reduces its virulence. YadA is a homotrimer of ca. 45-kDa subunits that are anchored to the outer membrane via their C-termini, while their N-termini form a globular head on top of a stalk; the 'lollipop'-shaped YadA structure covers the entire bacterial surface giving it hydrophobic properties. The yadA gene expression is induced at 37 °C by the temperature-dependent transcriptional activator LcrF. YadA is a multifaceted protein as revealed by its different biological properties. YadA⁺ bacteria bind to collagens, laminin, fibronectin, intestinal submucosa, mucus, and to hydrophobic surfaces like polystyrene. YadA⁺ bacteria autoagglutinate in stationary culture and also specifically agglutinate guinea pig red blood cells. YadA is also a potent serum resistance factor as it inhibits the classical pathway of complement. As invasin, it mediates low rate invasion to tissue culture cells. In a rat model of reactive arthritis YadA and specifically YadA-mediated collagen binding is necessary for Y. enterocolitica to induce the disease. Despite of this wealth of information or perhaps because of it, the in vivo role of YadA during infection remains still largely unresolved.

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YadA, the multifaceted Yersinia adhesin

Abstract

J. Biol. Chem.

FEMS Microbiol. Lett.

Cell

Microbes Infect.

Trends Microbiol.

Genetic analysis of a virulence plasmid from a serogroup 9 Yersinia enterocolitica strain: role of outer membrane protein P1 in resistance to human serum and autoagglutination.

Infect. Immun.

The Yersinia pseudotuberculosis adhesin YadA mediates intimate bacterial attachment to and entry into HEp-2 cells. Infect.

Immun.

Temperature-inducible outer membrane protein of Yersinia pseudotuberculosis and Yersinia enterocolitica is associated with the virulence plasmid.

Infect. Immun.

Molecular cloning of the temperature-inducible outer membrane protein 1 of Yersinia pseudotuberculosis

Infect. Immun.

Genetic evidence that the yopA gene-encoded Yersinia outer membrane protein Yop1 mediates inhibition of the anti-invasive effect of interferon

Infect. Immun.

Role of the YadA protein in prevention of opsonization of Yersinia enterocolitica by C3b molecules

Infect. Immun.

Yersinia enterocolitica

N. Engl. J. Med.

Functional mapping of the Yersinia enterocolitica adhesin YadA. Identification of eight NSVAIG–S motifs on the amino-terminal half of the protein involved in collagen binding.

Mol. Microbiol.

Binding to collagen by Yersinia enterocolitica and Yersinia pseudotuberculosis: evidence for yopA-mediated and chromosomally encoded mechanisms

J. Bacteriol.

Role of YadA-mediated collagen binding in arthritogenicity of Yersinia enterocolitica serotype O:8: Experimental studies with rats

Infect. Immun.

Role of YadA in arthritogenicity of Yersinia enterocolitica serotype O:8: Experimental studies with rats

Infect. Immun.

Reevaluation of the virulence phenotype of the inv yada double mutants of Yersinia pseudotuberculosis

Infect. Immun.

Determinants of invasion and survival of Yersinia enterocolitica in intestinal tissue. An in vivo study.

Med. Microbiol. Immunol.

Genetically manipulated virulence of Yersinia enterocolitica

Infect. Immun.

Structure and sequence analysis of Yersinia YadA and Moraxella UspAs reveal a novel class of adhesins.

EMBO J.

DNA repeats identify novel virulence genes in Haemophilus influenzae. Proc. Natl.

Acad. Sci. USA

Structural organization of virulence-associated plasmids of Yersinia pestis.

J. Bacteriol.

Determinants for the thermoinducible cell binding and plasmid-encoded cellular penetration detected in the absence of the Yersinia pseudotuberculosis invasin protein

Infect. Immun.

Relationship of virulence-associated autoagglutination to hemagglutinin production in Yersinia enterocolitica and Yersinia enterocolitica-like bacteria

Infect. Immun.

Temperature-inducible surface fibrillae associated with the virulence plasmid of Yersinia enterocolitica and Yersinia pseudotuberculosis

Infect. Immun.

Plasmid-mediated surface fibrillae of Yersinia pseudotuberculosis and Yersinia enterocolitica: Relationship to the outer membrane protein YOP1 and possible importance for pathogenesis

Infect. Immun.

Plasmid-associated cell surface charge and hydrophobicity of Yersinia enterocolitica

Infect. Immun.

Association of fibril structure formation with cell surface properties of Yersinia enterocolitica

Infect. Immun.

The UspA1 protein and a second type of UspA2 protein mediate adherence of Moraxella catarrhalis to human epithelial cells in vitro.

J. Bacteriol.

Role of the transcritional activator, VirF, and temperature in the expression of the pYV plasmid genes of Yersinia enterocolitica. Mol.

Microbiol.

Inhibition of human neutrophil chemiluminescence by plasmid-mediated outer membrane proteins of Yersinia enterocolitica

Infect. Immun.

Characterization of different oligomeric species of the Yersinia enterocolitica outer membrane protein YadA. Med. Microbiol.

Immunol.

Binding of Yersinia enterocolitica to rabbit intestinal brush border membranes, mucus, and mucin

Infect. Immun.