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Hyperlipidaemic effect of fish oil in Bio F1B hamsters

Published online by Cambridge University Press:  09 March 2007

Pujitha P. de Silva ,
Phillip J. Davis  and
Sukhinder Kaur Cheema
Pujitha P. de Silva
Affiliation:
Department of Biochemistry, Memorial University of Newfoundland, St John's NL, CanadaA1B 3X9
Phillip J. Davis
Affiliation:
Department of Biochemistry, Memorial University of Newfoundland, St John's NL, CanadaA1B 3X9
Sukhinder Kaur Cheema*
Affiliation:
Department of Biochemistry, Memorial University of Newfoundland, St John's NL, CanadaA1B 3X9
*
*Corresponding author: Dr Sukhinder Kaur Cheema, fax +1 709 737 2422, email skaur@mun.ca
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Abstract

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We investigated the dietary influence of low and high levels of fish oil, supplemented with or without dietary cholesterol, on the plasma lipoprotein profile in Bio F1B hamsters, a model susceptible to diet-induced hyperlipidaemia. The MIX diet, a diet supplemented with a mixture of lard and safflower-seed oil, was used as the control diet to maintain the saturated MUFA and PUFA levels similar to the fish-oil diet. The animals were fed the specific diets for 2 weeks and fasted for 14h before killing. The plasma from the animals fed high levels of fish oil was milky and rich in chylomicron-like particles. The plasma total cholesterol, VLDL- and LDL-cholesterol and -triacylglycerol concentrations were significantly higher, whereas HDL-cholesterol was lower in hamsters fed fish oil compared with the MIX-diet-fed hamsters. Increasing the amount of fat in the diet increased plasma lipids in both the fish-oil- and the MIX-diet-fed hamsters; however, this hyperlipidaemic effect of dietary fat level was greater in the hamsters fed the fish-oil diet. The hepatic lipid concentrations were not dramatically different between the fish-oil-fed and the MIX-diet-fed hamsters. However, the hepatic LDL-receptor mRNA levels were significantly low in the fish-oil-fed hamsters compared with the MIX-diet-fed hamsters. Increasing the amount of fish oil in the diet further decreased the hepatic LDL-receptor mRNA expression. It is concluded that F1B hamsters are susceptible to fish-oil-induced hyperlipidaemia, especially at high fat levels, and this increase is partially explained by the inhibition of hepatic LDL-receptor mRNA expression.

Keywords

Fish oilBio F1B hamstersLipoproteinsChylomicrons
Type
Research Article
Information
British Journal of Nutrition , Volume 91 , Issue 3 , March 2004 , pp. 341 - 349
Copyright
Copyright © The Nutrition Society 2004

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