Hostname: page-component-8448b6f56d-42gr6 Total loading time: 0 Render date: 2024-04-19T16:54:37.028Z Has data issue: false hasContentIssue false
  • English
  • Français

Dietary intervention with the probiotics Lactobacillus acidophilus 145 and Bifidobacterium longum 913 modulates the potential of human faecal water to induce damage in HT29clone19A cells

Published online by Cambridge University Press:  09 March 2007

Daniela L Oberreuther-Moschner ,
Gerhard Jahreis ,
Gerhard Rechkemmer  and
Beatrice L Pool-Zobel
Daniela L Oberreuther-Moschner
Affiliation:
Institute of Nutritional Physiology, Federal Research Centre for Nutrition, Haid-und-Neu-Straβe 9, 76131 Karlsruhe, Germany
Gerhard Jahreis
Affiliation:
Department of Nutritional Physiology, Institute for Nutrition, Friedrich-Schiller University, Dornburger Straβe 25, D-07743 Jena, Germany
Gerhard Rechkemmer
Affiliation:
Institute of Nutritional Physiology, Federal Research Centre for Nutrition, Haid-und-Neu-Straβe 9, 76131 Karlsruhe, Germany
Beatrice L Pool-Zobel*
Affiliation:
Institute of Nutritional Physiology, Federal Research Centre for Nutrition, Haid-und-Neu-Straβe 9, 76131 Karlsruhe, Germany
*
*Corresponding author: Professor Beatrice L. Pool-Zobel, fax +49 3641 949672, email b8pobe@uni-jena.de
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Probiotics reduce the risk of colon cancer by inhibiting carcinogen-induced DNA damage in animals, but there are no analogous data in human subjects. To enhance knowledge of the effects of probiotics in human subjects, we have investigated the genotoxicity of faecal water after dietary intervention with standard yoghurt or with probiotic yoghurt, which included the strains Lactobacillus acidophilus 145 and Bifidobacterium longum 913. Faeces were collected from nine healthy volunteers after intervention with probiotic yoghurt or standard yoghurt. Faecal water was isolated and incubated with human colon tumour cells HT29clone19A. DNA strand breaks, oxidised DNA bases and damage after challenge with H2O2 were determined by micro-gel-electrophoresis. Faecal water was genotoxic in comparison with NaCl, but protected against H2O2-induced DNA strand breaks. The intervention with probiotic yoghurt significantly lowered faecal water genotoxicity compared with standard yoghurt. However, probiotic intervention also increased oxidative damage; this either reflected prooxidative activity or stimulation of endogenous defence systems. Altogether, the balance of effects favoured protection, since faecal water from the probiotic group reduced overall genetic damage. Thus, there was a reduction of strand break-inducing compounds in human faeces after dietary intervention with probiotic bacteria. This protection reflected results from previous studies in carcinogen-exposed animals where probiotics reduced DNA damage in colon cells.

Keywords

Faecal waterComet assayOxidative DNA damage
Type
Research Article
Information
British Journal of Nutrition , Volume 91 , Issue 6 , June 2004 , pp. 925 - 932
Copyright
Copyright © The Nutrition Society 2004

References

Augeron, C & Laboisse, CL (1984) Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Res 44, 3961–3969.Google Scholar
Benno, Y & Mitsuoka, T (1992) Impact of Bifidobacterium longum on human fecal microflora. Microbiol Immunol 36, 683–694.CrossRefGoogle ScholarPubMed
Bolognani, F, Rumney, CJ & Rowland, IR (1997) Influence of carcinogen binding by lactic acid-producing bacteria on tissue distribution and in vivo mutagenicity of dietary carcinogens. Food Chem Toxicol 35, 535–545.CrossRefGoogle ScholarPubMed
Bottrill, K (1998) The use of biomarkers as alternatives to current animal tests on food chemicals. Altern Lab Anim 26, 421–480.CrossRefGoogle ScholarPubMed
Branca, F, Hanley, AB, Pool-Zobe, BL & Verhagen, H (2001) Biomarkers in disease and health. Br J Nutr 86 S55–S92.CrossRefGoogle ScholarPubMed
Brashears, MM, Gilliland, SE & Buck, LM (1998) Bile salt deconjugation and cholesterol removal from media by Lactobacillus casei. J Dairy Sci 81, 2103–2110.CrossRefGoogle ScholarPubMed
Bruce, WR, Varghese, AJ, Furrer, R & Land, PC (1977) A mutagen in the feces of normal humans. In Origins of Human Cancer, Cold Spring Harbor Conferences of Cell Proliferation, Book C:Human Risk Assessment, pp. 1641–1646 [Hiatt, HH, Watson, JD and Winsten, JA, editors]. New York: Cold Spring Harbor.Google Scholar
Bruce, WR, Varghese, AJ, Wang, S 7 Dion, P (1979) The endogenous production of nitroso compounds in the colon and cancer at this site. In Naturally Occuring Carcinogens – Mutagens and Modulators of Carcinogenesis, pp. 221–228 [Miller, EC, editor]. Baltimore, MD: University Park Press.Google Scholar
Curnutte, JT & Babior, BM (1987) Chronic granulomatous diseases. Adv Hum Genet 16, 229–297.CrossRefGoogle Scholar
de Kok, TMCM & Van Maanen, JMS (2000) Evaluation of fecal mutagenicity and colorectal cancer risk. Mutat Res 463, 53–101.CrossRefGoogle ScholarPubMed
Duthie, SJ, Ma, A, Ross, MA & Collins, AR (1996) Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes. Cancer Res 56, 1291–1295.Google ScholarPubMed
Ehrich, M, Aswell, JE, Van Tassell, RL & Wilkins, TD (1979) Mutagens in the feces of three South-African populations at different levels of risk for colon cancer. Mutat Res 64, 231–240.CrossRefGoogle Scholar
Engesser, DM & Hammes, WP (1994) Non-heme catalase activity of lactic acid bacteria. System Appl Microbiol 17, 11–19.CrossRefGoogle Scholar
Ferguson, LR, Alley, PG & Gribben, BM (1985) DNA-damaging activity in ethanol-soluble fractions of feces from New Zealand Groups at varying risks of colorectal cancer. Nutr Cancer 5, 93–103.CrossRefGoogle Scholar
Ferlay, J, Bray, F, Pisani, P & Parkin, DM (2001) GLOBOCAN 2000: Cancer Incidence, Mortality and Prevalence Worldwide. IARC Cancer Base No. 5. Lyon: IARC Press.Google Scholar
Fernandes, CF & Shahani, KM (1990) Anticarcinogenic and immunological properties of dietary Lactobacilli. Food Prot 53, 704–710.CrossRefGoogle ScholarPubMed
Fuller, R (1989) Probiotics in man and animals. J Appl Bacteriol 66, 365–378.Google Scholar
Glinghammar, B, Venturi, M, Rowland, IR & Rafter, JJ (1997) Shift from a dairy product-rich to a dairy product-free diet: influence on cytotoxicity and genotoxicity of fecal water – potential risk factors for colon cancer. Am J Clin Nutr 66, 1277–1282.CrossRefGoogle ScholarPubMed
Goldin, B & Gorbach, SL (1984 a) The effect of milk and Lactobacillus feeding on human intestinal bacterial enzyme activity. Am J Clin Nutr 39, 756–761.CrossRefGoogle ScholarPubMed
Goldin, B & Gorbach, SL (1984 b) The effect of oral administration on Lactobacillus and antibiotics on intestinal bacteria activity and chemical induction of large bowel tumors. Dev Ind Microbiol 25, 139–150.Google Scholar
Goldin, B, Gorbach, SL, Saxelin, M, Barakat, S, Gualtiere, L & Salminen, S (1992) Survival of Lactobacillus species (strain GG) in human gastrointestinal tract. Dig Dis Sci 37, 121–128.CrossRefGoogle ScholarPubMed
Hayatsu, H & Hayatsu, T (1994) Suppressing effect of Lactobacillus casei administration on the urinary mutagenicity arising from ingestion of fried ground beef in the human. Cancer Lett 73, 2–3.Google Scholar
Kieβling, G, Schneider, J & Jahreis, G (2002) Long-term consumption of fermented dairy products over 6 months increases HDL cholesterol. Eur J Clin Nutr 56, 843–849.CrossRefGoogle Scholar
Kuhnlein, HV & Kuhnlein, U (1981) Mutagens in feces from subjects on controlled formula diets. Nutr Cancer 2, 119–124.CrossRefGoogle Scholar
Kuhnlein, HV, Kuhnlein, U & Bell, PA (1983) The effect of short-term dietary modification on human fecal mutagenic activity. Mutat Res 113, 1–12.CrossRefGoogle ScholarPubMed
Kuhnlein, U, Bergstrom, D & Kuhnlein, H (1981) Mutagens in feces from vegetarians and non-vegetarians. Mutat Res 85, 1–12.CrossRefGoogle ScholarPubMed
Lechner, S, Müller-Ladner, U, Schlottmann, K, Jung, B, McClelland, M, Ruschoff, J, Welsh, J, Scholmerich, J & Kullmann, F (2002) Bile acids mimic oxidative stress induced upregulation of thioredoxin reductase in colon cancer cell lines. Carcinogenesis 23, 1281–1288.CrossRefGoogle ScholarPubMed
Lidbeck, A, Övervik, E, Rafter, J, Nord, CE & Gustafsson, JA (1992) Effect of Lactobacillus acidophilus supplements on mutagen excretion in faeces and urine in humans. Microb Ecol Health Dis 5, 59–67.Google Scholar
Liegibel, U, Abrahamse, SL, Pool-Zobel, BL & Rechkemmer, G (2000) Application of confocal laser scanning microscopy to detect oxidative stress in human colon cells. Free Radic Res 32, 535–547.CrossRefGoogle ScholarPubMed
McKelvey-Martin, VJ, Green, MHL, Schmezer, P, Pool-Zobel, BL, De Méo, MP & Collins, AR (1993) The single cell gel electrophoresis assay (comet assay): a European review. Mutat Res 288, 47–63.CrossRefGoogle ScholarPubMed
Marteau, P, Pochart, P,Flourié, B, Pellier, P, Santos, L, Desjeux, JF & Rambaud, JC (1990) Effect of chronic ingestion of a fermented dairy product containing Lactobacillus acidophilus and Bifidobacterium longum on metabolic activities of the colonic flora in humans. Am J Clin Nutr 52, 658–688.CrossRefGoogle Scholar
Nader, CJ, Potter, JD & Weller, RA (1981) Diet and DNA-modifying activity in human fecal extracts. Nutr Rep Int 23, 113–117.Google Scholar
Oβwald, K, Becker, TW, Grimm, M, Jahreis, G & Pool-Zobel, BL (2000) Inter- and intra-individual variation of faecal water – genotoxicity in human colon cells. Mutat Res 472, 59–70.Google Scholar
Perera, FP (1996) Molecular epidemiology: Insights into cancer susceptibility, risk assessment, and prevention. J Natl Cancer Inst 88, 496–509.CrossRefGoogle ScholarPubMed
Pool-Zobel, BL, Abrahamse, SL, Collins, AR, Kark, W, Gugler, R, Oberreuther, D, Siegel, EG, Treptow-Van Lishaut, S & Rechkemmer, G (1999) Analysis of DNA strand breaks, oxidized bases, and glutathion S-transferase P1 in human colon cells from biopsies. Cancer Epidemiol Biomarkers Prev 8, 609–614.Google ScholarPubMed
Pool-Zobel, BL, Bertram, B, Knoll, M, Lambertz, R, Neudecker, C, Schillinger, U, Schmezer, P & Holzapfel, WH (1993) Antigenotoxic properties of lactic acid bacteria in vivo in the gastrointestinal tract of rats. Nutr Cancer 20, 271–281.CrossRefGoogle ScholarPubMed
Pool-Zobel, BL, Neudecker, C, Domizlaff, I, Ji, S, Schillinger, U, Rumney, C, Moretti, M, Villarini, I, Scassellati-Sforzolini, R & Rowland, I (1996) Lactobacillus- and bifidobacterium-mediated antigenotoxicity in the colon of rats. Nutr Cancer 26, 365–380.CrossRefGoogle ScholarPubMed
Pool-Zobel, BL, Van Loo, J, Rowland, IR & Roberfroid, MB (2002) Experimental evidences on the potential of prebiotic fructans to reduce the risk of colon cancer. Br J Nutr 87, S273–S281.CrossRefGoogle ScholarPubMed
Potter, JD (1999) Colorectal cancer: molecules and populations. J Natl Cancer Inst 91, 916–932.CrossRefGoogle ScholarPubMed
Reddy, BS (1998) Prevention of colon cancer by pre and probiotics: evidence from laboratory studies. Br J Nutr 80, S219–S223.CrossRefGoogle ScholarPubMed
Rieger, MA, Parlesak, A, Pool-Zobel, BL, Rechkemmer, G & Bode, Ch (1999) A diet high in fat and meat but low in dietary fibre increases the genotoxic potential of ‘faecal water’. Carcinogenesis 20, 2311–2316.CrossRefGoogle ScholarPubMed
Rowland, IR (1995) Toxicology of the colon: role of the intestinal microflora. In Human Colonic Bacteria: Role in Nutrition, Physiology and Pathology, pp. 155–174 [Gibso, GR and Macfarlane, GT, editors].London: CRD Press.Google Scholar
Sandström, B (1965) Studies on cells from liver tissue cultivated in vitro. Exp Cell Res 37, 552–568.CrossRefGoogle Scholar
Schiffrin, EJ, Rochat, F, Link-Amster, H, Aeschlimann, JM & Donnet-Hughes, A (1995) Immunomodulation of human blood cells following the ingestion of lactic acid bacteria. J Dairy Sci 78, 491–497.CrossRefGoogle ScholarPubMed
Singh, J, Rivenson, A, Tomita, M, Shimamura, S, Ishibashi, N & Reddy, BS (1997) Bifidobacterium longum, a lactic acid-producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis. Carcinogenesis 18, 833–841.CrossRefGoogle ScholarPubMed
Terry, P, Giovannucci, E, Michels, KB, Bergkvist, L, Hansen, H, Holmberg, L & Wolk, A (2001) Vegetables, dietary fiber, and risk of colorectal cancer. J Natl Cancer Inst 93, 525–533.CrossRefGoogle ScholarPubMed
Varghese, AJ, Land, PC, Furrer, R 7 Bruce, WR (1978) Non-volatile N -nitroso compounds in human faeces. In Environmental Aspects of N-Nitroso Compounds, pp. 257–264 [Walker, EA, Griciute, L, Castegnaro, M, Lyle, R and Davis, W, editors]. Lyon: International Agency for Research on Cancer.Google Scholar
Venturi, M, Hambly, RJ, Glinghammar, B, Rafter, JJ & Rowland, IR (1997) Genotoxic activity in human faecal water and the role of bile acids: a study using the alkaline comet assay. Carcinogenesis 18, 2353–2359.CrossRefGoogle ScholarPubMed
Wollowski, I, Ji, ST, Bakalinsky, AT, Neudecker, C & Pool-Zobel, BL (1999) Bacteria used for the production of yogurt inactivate carcinogens and prevent DNA damage in the colon of rats. J Nutr 129, 77–82.CrossRefGoogle ScholarPubMed
Wollowski, I, Rechkemmer, G & Pool-Zobel, BL (2001) Protective role of probiotics and prebiotics in colon cancer. Am J Clin Nutr 73, 451–455.CrossRefGoogle ScholarPubMed
World Cancer Research Fund (1997) Food, nutrition and the prevention of cancer: a global perspective. Washington, DC: American Institute for Cancer Research.Google Scholar
World Cancer Research Fund, American Institute for Cancer Research & Deutsches Institut für Ernährungsforschung (1999) Krebsprävention durch Ernährung: Forschung, Daten, Begründungen, Empfehlungen (Cancer Prevention by Nutrition: Research, Data, Reasons, Recommendations), pp. 1–29. Potsdam-Rehbrücke: World Cancer Research Fund, American Institute for Cancer Research, Deutsches Institut für Ernährungsforschung.Google Scholar