Elsevier

Pathology

Volume 41, Issue 3, April 2009, Pages 229-233
Pathology

Mac-2 binding protein and galectin-3 expression in mucinous tumours of the ovary: an annealing control primer system and immunohistochemical study

https://doi.org/10.1080/00313020902756279Get rights and content

Summary

Aim

We used a new differential display method, the annealing control primer (ACP) system, to analyse the differentially expressed genes in mucinous ovarian tumours. To verify the corresponding target gene, immunohistochemical staining was performed on various epithelial tumours of the ovary.

Methods and Results

The ACP-based reverse transcriptase-polymerase chain reaction revealed that 21 genes were upregulated in the mucinous ovarian adenoma and 14 genes were upregulated in the mucinous ovarian carcinoma. Among them, we selected one upregulated gene, the Mac-2 binding protein (Mac-2 BP), and verified the expression of the Mac-2 BP and its ligand, galectin-3, in a variety of epithelial ovarian tumours by immunohistochemistry. Positive expression of the Mac-2 BP was significantly higher in the mucinous ovarian tumours compared to the other epithelial tumours. Mac-2 BP expression was significantly increased in the borderline and malignant tumours compared to the benign tumours. Galectin-3 expression was more frequent in clear cell carcinomas, serous tumours and mucinous tumours than in endometrioid and transitional tumours. However, there were no differences in galectin-3 expression in comparisons among benign, borderline and malignant mucinous and serous tumours.

Conclusion

These data indicate that the Mac-2 BP may play a role in the development and progression of mucinous ovarian tumours.

References (25)

  • I.T. Hwang et al.

    Annealing control primer system for improving specificity of PCR amplification

    Biotechniques

    (2003)
  • S. Iacobelli et al.

    Detection of antigens recognized by a novel monoclonal antibody in tissue and serum from patients with breast cancer

    Cancer Res

    (1986)
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