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Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

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Abstract

The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.

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Authors and Affiliations

  1. The Macfarlane Burnet Institute for Medical Research and Public Health, 85 Commercial Rd., 3004, Melbourne, Victoria, Australia

    Melissa J. Churchill, Paul R. Gorry, Daniel Cowley, Luxshimi Lal, Secondo Sonza, Katherine A. Thompson & Steven L. Wesselingh

  2. Department of Medicine, Monash University, Melbourne, Victoria, Australia

    Paul R. Gorry & Steven L. Wesselingh

  3. Department of Microbiology, Monash University, Melbourne, Victoria, Australia

    Secondo Sonza

  4. Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia

    Paul R. Gorry & Damian F. J. Purcell

  5. Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA, USA

    Dana Gabuzda

  6. Department of Neurology, Harvard Medical School, Boston, MA, USA

    Dana Gabuzda

  7. Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Justin C. McArthur & Carlos A. Pardo

Authors
  1. Melissa J. Churchill
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  2. Paul R. Gorry
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  3. Daniel Cowley
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  4. Luxshimi Lal
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  5. Secondo Sonza
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  6. Damian F. J. Purcell
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  7. Katherine A. Thompson
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  8. Dana Gabuzda
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  9. Justin C. McArthur
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  10. Carlos A. Pardo
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  11. Steven L. Wesselingh
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Corresponding author

Correspondence to Paul R. Gorry.

Additional information

This study was supported by grants from the Australian National Health and Medical Research Council (NHMRC) to S.L.W. (358399), P.R.G. (251520) and S.S. (281209), grants from NIH/NIAID (R21-AI054207) to P.R.G., NIH/NINDS (NS44807 and NS049465) to J.C.M., NIH/NIDA (K08DA16160) to C.A.P., and NIH/NS (R01-NS37277) to D.G, and a grant from the Clive and Vera Ramaciotti Foundation to D.F.J.P. P.R.G. is a recipient of an NHMRC R. Douglas Wright Biomedical Career Development Award.

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Churchill, M.J., Gorry, P.R., Cowley, D. et al. Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues. Journal of NeuroVirology 12, 146–152 (2006). https://doi.org/10.1080/13550280600748946

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  • DOI: https://doi.org/10.1080/13550280600748946

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