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The Effect of Blood on the Antiviral Activity of Sodium Hypochlorite, a Phenolic, and a Quaternary Ammonium Compound

Published online by Cambridge University Press:  02 January 2015

David J. Weber ,
Susan L. Barbee ,
Mark D. Sobsey  and
William A Rutala
David J. Weber*
Affiliation:
Division of Infectious Diseases, University of North Carolina (UNC) School of Medicine, UNC School of Public Health, Chapel Hill, North Carolina Department of Hospital Epidemiology, UNC Hospitals, UNC School of Public Health, Chapel Hill, North Carolina Departments of Epidemiology, UNC School of Public Health, Chapel Hill, North Carolina
Susan L. Barbee
Affiliation:
Division of Infectious Diseases, University of North Carolina (UNC) School of Medicine, UNC School of Public Health, Chapel Hill, North Carolina
Mark D. Sobsey
Affiliation:
Environmental Sciences, UNC School of Public Health, Chapel Hill, North Carolina
William A Rutala
Affiliation:
Division of Infectious Diseases, University of North Carolina (UNC) School of Medicine, UNC School of Public Health, Chapel Hill, North Carolina Department of Hospital Epidemiology, UNC Hospitals, UNC School of Public Health, Chapel Hill, North Carolina
*
547 Burnett-Womack Building, CB #7030, Division of Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7030
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Abstract

Objective:

To assess the virucidal activity of three disinfectants (sodium hypochlorite, a phenolic, and a quaternary ammonium compound) in the presence and absence of blood.

Methods:

Disinfectants at varying concentrations (hypochlorite: 5,000, 500, or 50 ppm; phenolic: 1:10 or 1:128 dilution; quaternary ammonium compound: 1:10 or 1:128 dilution) were added to either saline or whole blood (final concentration, 80% or 20% blood) and mixed. Test organisms included an attenuated vaccine strain of poliovirus type 1 (prototype for relatively resistant hydrophilic viruses) and herpes simplex virus (HSV) type 1 (prototype for relatively susceptible lipophilic viruses). Virus was added to create a viral-blood suspension. Viral survival was tested at room temperature at the following times: 0, 15 seconds, 30 seconds, 1 minute, 2 minutes, 5 minutes, and 10 minutes. A neutralizer stopped the reaction, and virus was assayed using a plaque technique.

Results:

In the absence of blood, complete inactivation of HSV was achieved within 30 seconds with 5,000 (1:10 dilution of bleach) and 500 (1:100 dilution of bleach) ppm chlorine, 1:10 and 1:128 diluted phenolic (use dilution), and 1:10 and 1:128 diluted quaternary ammonium compound (use dilution). In the presence of 80% blood, only 5,000 ppm hypochlorite, 1:10 phenolic, and 1:10 or 1:128 quaternary ammonium compound were effective. In the absence of blood, complete inactivation of polio was achieved within 30 seconds by 5,000 and 500 ppm chlorine and 1:10 quaternary ammonium compound. In the presence of 80% blood, no solution tested was capable of completely inactivating poliovirus within 10 minutes.

Conclusions:

Our data suggest that, in the absence of visible blood, environmental surfaces may be disinfected with a diluted hypochlorite solution (1:10 or 1:100), a phenolic, or a quaternary ammonium compound. Based on our studies using HSV, which has similar susceptibilities to disinfectants as human immunodeficiency virus (HIV), phenolics at their use dilution and 1:100 diluted hypochlorite are unlikely to inactivate HIV or hepatitis B virus reliably in the presence of blood. Hypochlorite at a final concentration of 5,000 ppm (1:10 dilution) should be used to decontaminate blood spills, but, even after decontamination, care should be used to avoid sharps injuries.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 20 , Issue 12 , December 1999 , pp. 821 - 827
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1999

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References

1. Jagger, J, Hunt, EH, Brand-Elnaggar, J, Pearson, RD. Rates of needle-stick injury caused by various devices in a university hospital. N Engl J Med 1988;319:284–288.Google Scholar
2. Centers for Disease Control and Prevention. Public Health Service guidelines for the management of health-care worker exposures to HIV and recommendations for postexposure prophylaxis. MMWR 1998;47(RR-7):1–57.Google Scholar
3. Grady, GF, Lee, VA, Prince, AM, Gitnick, GL, Fawaz, KA, Vyas, GN, et al. Hepatitis B immune globulin for accidental exposures among medical personnel: final report of a multicenter controlled trial. J Infect Dis 1978;138:625–638.Google Scholar
4. Hoofnagle, JH, Seeff, LB, Bales, ZB, Wright, EC, Zimmerman, HJ. Passive-active immunity from hepatitis B immune globulin. Reanalysis of a Veterans' Administration cooperative study of needle-stick hepatitis. The Veterans' Administration Cooperative Study Group. Ann Intern Med 1979;91:813–818.CrossRefGoogle ScholarPubMed
5. Werner, BG, Grady, GF. Accidental hepatitis-B-surface-antigen-positive inoculations. Use of e antigen to estimate infectivity. Ann Intern Med 1982;97:367–369.Google Scholar
6. Centers for Disease Control and Prevention. Recommendations for prevention and control of hepatitis C virus (HCV) infection and HCV-related chronic disease. MMWR 1998;47(RR-19):19:1–39.Google Scholar
7. Ippolito, G, Puro, V, Petrosillo, N, De Carli, G, Micheloni, G, Magliano, E. Simultaneous infection with HIV and hepatitis C virus following occupational conjunctival blood exposure. JAMA 1998;280:28–29.Google Scholar
8. Sartori, M, La Terra, G, Aglietta, M, Manzin, A, Navino, C, Verzetti, G. Transmission of hepatitis C via blood splash into conjunctiva. Scand J Infect Dis 1993;25:270–271.Google Scholar
9. Occupational Safety and Health Administration. Occupational exposure to bloodborne pathogens. Final rule. Federal Register December 6, 1991;56:64175–64182.Google Scholar
10. Centers for Disease Control and Prevention. Guidelines for prevention of transmission of human immunodeficiency virus and hepatitis B virus to health-care and public-safety workers. MMWR 1989;38(S-6);1–37.Google Scholar
11. Rutala, WA, the APIC Guidelines Committee. APIC guideline for selection and use of disinfectants. Am J Infect Control 1996;24:313–342.Google Scholar
12. Klein, M, DeForest, A. The inactivation of viruses by germicides. Chemical Specialists Manufacturing Association Proceedings 1963;49:116–118.Google Scholar
13. American Public Health Association. Standard Methods for the Examination of Water and Wastewater. 16th ed. Washington, DC: American Public Health Association; 1985. Method #408.D.Google Scholar
14. Spire, B, Barre-Sinoussi, F, Montagnier, L, Chermann, JC. Inactivation of lymphadenopathy associated virus by chemical disinfectants. Lancet 1984;2:899–901.Google Scholar
15. Martin, LS, McDougal, JS, Loskoski, SL. Disinfection and inactivation of the human T lymphotropic virus type III/lymphadenopafhy-associated virus. J Infect Dis 1985;152:400–403.Google Scholar
16. Bloomfield, SF, Smith-Burchnell, CA, Dalgleish, AG. Evaluation of hypochlorite-releasing disinfectants against the human immunodeficiency virus (HIV). J Hosp Infect 1990;15:273–278.CrossRefGoogle ScholarPubMed
17. van Bueren, J, Larkin, DP, Simpson, RA. Inactivation of human immunodeficiency virus type 1 by alcohols. J Hosp Infect 1994;28:137–148.CrossRefGoogle ScholarPubMed
18. Flynn, N, Jain, S, Keddie, EM, Carlson, JR, Jennings, MB, Haverkos, HW, et al. In vitro activity of readily available household materials against HIV-1: is bleach enough? J Acquir Immune Defic Syndr 1994;7:747–753.Google Scholar
19. Druce, JD, Jardine, D, Locarnini, SA, Birch, CJ. Susceptibility of HIV to inactivation by disinfectants and ultraviolet light. J Hosp Infect 1995;30:167–180.Google Scholar
20. Tsiquaye, KN, Barnard, J. Chemical disinfection of duck hepatitis B virus: a model for inactivation of infectivity of hepatitis B virus. J Antimicrob Chemother 1993;32:313–323.CrossRefGoogle Scholar
21. Prince, DL, Prince, HN, Thraenhart, O, Muchmore, E, Bonder, E, Pugh, J. Methodological approaches to disinfection of human hepatitis B virus. J Clin Microbiol 1993;31:3296–3304.Google Scholar
22. Hanson, PJV, Gor, D, Jeffries, DJ, Collins, JV. Chemical inactivation of HIV on surfaces. BMJ 1989;298:862–864.Google Scholar
23. Agolini, G, Russo, A, Clementi, M. Effect of phenolic and chlorine disinfectants on hepatitis C virus binding and infectivity. Am J Infect Control 1999;27:236–239.Google Scholar
24. Tjotta, E, Hungnes, O, Grinde, B. Survival of HIV-1 activity after disinfection, temperature and pH changes, or drying. J Med Virol 1991;35:223–227.CrossRefGoogle ScholarPubMed
25. Resnick, L, Veren, K, Salahuddin, SZ, Tondreau, S, Markham, P. Stability and inactivation of HTLV-III/LAV under clinical and laboratory environments. JAMA 1986:255:1887–1891.Google Scholar
26. Bond, WW, Favero, MS, Petersen, NJ, Gravelle, CR, Ebert, JW, Maynard, JE. Survival of hepatitis B virus after drying and storage for one week. Lancet 1981;1:550–551.CrossRefGoogle ScholarPubMed
27. Bond, WW, Favero, MS, Petersen, NJ, Ebert, JW. Inactivation of hepatitis B virus by intermediate-to-high level disinfectant chemicals. J Clin Microbiol 1983;18:535–538.CrossRefGoogle ScholarPubMed
28. Rutala, WA, Cole, EC. Ineffectiveness of hospital disinfectants against bacteria: a collaborative study. Infect Control 1987;8:501–506.Google Scholar