European Journal of Gastroenterology & Hepatology

Accession Number<strong>00042737-200105000-00005</strong>.
AuthorWerling, Klara a; Szentirmay, Zoltan b; Szepesi, Agota c; Schaff, Zsuzsa d; Szalay, Ferenc e; Szabo, Zsuzsa f; Telegdy, Laszlo f; David, Karoly g; Stotz, Gyula g; Tulassay, Zsolt a
Institution(a)Second Department of Medicine, Joint Research University of the Hungarian Academy of Sciences, Budapest, (b)National Institute of Oncology, Budapest, (c)First Institute of Pathology and Experimental Cancer Research, Budapest,(d)Second Institute of Pathology Budapest, (e)First Department of Medicine, Semmelweis University Medical School, Budapest, (f)Szent Laszlo Hospital Budapest, and (g)Central Hospital of the Ministry of Internal Affairs, Budapest, Hungary
TitleHepatocyte proliferation and cell cycle phase fractions in chronic viral hepatitis C by image analysis method.[Article]
SourceEuropean Journal of Gastroenterology & Hepatology. 13(5):489-493, May 2001.
AbstractObjective: Chronic hepatitis is characterized by necrosis of liver cells, accompanied by an inflammatory reaction and compensatory cell proliferation. The interaction of the core and non-structural proteins of hepatitis C virus (HCV) with several cellular factors suggests that cell proliferation may be influenced by HCV. The aim of this study was to investigate hepatocyte proliferation and DNA ploidy patterns in patients with chronic viral hepatitis C (CH-C) compared with chronic non-viral hepatitis (CH-N), using a TV image analysis method.

Methods: The DNA index (DI) and cell phase fractions (G1, S, G2) were measured by means of digital picture analysis method on nuclear suspensions of Feulgen stained hepatocytes. Cells were taken from the liver biopsy specimens of 71 patients with CH-C and 24 patients with CH-N. Twenty-six normal liver samples were used as controls.

Results: Significantly higher G1 (94 +/- 4) and lower S (3.56 +/- 3.16) phase fractions were measured in CH-C compared with CH-N (G1, 90 +/- 6; S, 6.4 +/- 5.99). The DI of moderate (1.12 +/- 0.05) and severe (1.12 +/- 0.05) CH-C showed near-aneuploid DNA content, while diploidy (DI < 1.10) was detected in cases of CH-N.

Conclusion: The higher G1 and lower S cell cycle phase fractions in CH-C reflect decreased hepatocyte proliferation compared with CH-N. The near-aneuploid DNA content of the HCV-infected liver samples may be a sign of increased genetic instability, which may contribute to the carcinogenic potential of HCV.

(C) 2001 Lippincott Williams & Wilkins, Inc.