1887

Abstract

Rickettsiae are classified in the order and have been included in the α subclass of the class on the basis of 16S rRNA gene sequence comparison. To estimate the evolutionary forces that have shaped the members of the spotted fever group (SFG) rickettsiae, the gene (apart from the tandem repeat units), encoding an antigenic high-molecularmass membrane protein specific for the group, was amplified and sequenced from 21 isolates. The phylogenetic relationships between SFG rickettsiae were inferred from the comparison of both the gene and derived protein sequences, using the parsimony, neighbour-joining and maximum-likelihood methods. Three strongly supported phylogenetic sub-groups were distinguished: first, the conorii complex (R. conorii Malish, R. conorii M1, R. conorii Moroccan, Indian tick typhus, Astrakhan fever rickettsia and Israeli tick typhus rickettsia); second, a cluster including , strain S, and ‘’; and, third, a cluster including , Bar 29 and and Thai tick typhus rickettsia did not cluster with any other Rickettsia species. To test whether positive selection was responsible for sequences diversity, rates of synonymous and nonsynonymous nucleotide substitutions were compared for alleles and indicated that this gene is undergoing neutral evolution.

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1998-07-01
2024-03-28
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References

  1. Anacker R. L., McDonald G. A., List R. H., Mann R. E. 1987; Neutralizing activity of monoclonal antibodies to heat-sensitive and heat-resistant epitopes of Rickettsia rickettsii surface proteins. Infect Immun 55:825–827
    [Google Scholar]
  2. Anderson B. E., McDonald G. A., Jones D. C., Regnery R. L. 1990; A protective protein antigen of Rickettsia rickettsii has tandemly repeated, near-identical sequences. Infect Immun 58:2760–2769
    [Google Scholar]
  3. Andrew R., Bonnin J. M., Williams S. 1946; Tick typhus in North Queensland. Med J Aust 2:253–258
    [Google Scholar]
  4. Babalis T., Tselentis Y, Roux V., Psaroulaki A., Raoult D. 1994; Isolation and identification of a rickettsial strain related to Rickettsia massiliae in greek ticks. Am J Trop Med Hyg 50:365–372
    [Google Scholar]
  5. Bacellar F., Regnery R. L., Nuncio M. S., Filipe A. R. 1995; Genotypic evaluation of rickettsial isolates recovered from various species of ticks in Portugal. Epidemiol Infect 114:169–178
    [Google Scholar]
  6. Beati L., Raoult D. 1993; Rickettsia massiliae sp. nov., a new spotted fever group rickettsia. Int J Syst Bacteriol 43:839–840
    [Google Scholar]
  7. Beati L., Roux V., Ortuno A., Castella J., Segura-Porta F., Raoult D. 1996; Phenotypic and genotypic characterization of spotted fever group rickettsiae isolated from Catalan Rhipicephalus sanguineus ticks. J Clin Microbiol 34:2688–2694
    [Google Scholar]
  8. Beati L., Meskini M., Thiers B., Raoult D. 1997; Rickettsia aeschlimannii sp. nov.: a new spotted fever group rickettsia associated with Hyalomma marginatum ticks. Int J Syst Bacteriol 47:548–554
    [Google Scholar]
  9. Bell E. J., Stoenner H. G. 1960; Immunologic relationships among the spotted fever group of rickettsiae determined by toxin neutralisation tests in mice with convalescent animal serums. J Immunol 84:171–210
    [Google Scholar]
  10. Bell E. J., Kohls G. M., Stoenner H. G., Lackman D. B. 1963; Non pathogenic rickettsiae related to the spotted fever group isolated from ticks, Dermacentor variabilis and Dermacentor andersoni from Eastern Montana. J Immunol 90:770–781
    [Google Scholar]
  11. Bella F., Espero E., Uriz S., Serrano J. A., Allegre M. A., Tort J. 1991; Randomized trial of 5-day rifampin versus 1-day doxycycline therapy for MSF. J Infect Dis 164:433–434
    [Google Scholar]
  12. Birtles R. J., Raoult D. 1996; Comparison of partial citrate synthase gene (gltA) sequences for phylogenetic analysis of Bartonella species. Int J Syst Bacteriol 46:891–897
    [Google Scholar]
  13. Birtles R. J., Harrison T. G., Saunders N. A., Molyneux D. H. 1995; Proposals to unify the genera Grahamella and Bartonella, with descriptions of Bartonella talpae comb, nov., Bartonella peromysci comb, nov., and three new species, Bartonella grahamii sp. nov., Bartonella taylorii sp. nov., and Bartonella doshiae sp. nov. Int J Syst Bacteriol 45:1–8
    [Google Scholar]
  14. Black W. C. IV, Piesman J. 1994; Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences. Proc Natl Acad Sci USA 91:10034–10038
    [Google Scholar]
  15. Brenner D., J„ O'Connor S. P., Winkler H. H., Steigerwalt A. G. 1993; Proposals to unify the genera Bartonella and Rochalimaea, with descriptions of Bartonella quintana comb, nov., Bartonella vinsonii comb, nov., Bartonella henselae comb, nov., and Bartonella elizabethae comb, nov., and to remove the family Bartonellaceae from the order Rickettsiales. Int J Syst Bacteriol 43:777–786
    [Google Scholar]
  16. Brown J. K. M. 1994; Bootstrap hypothesis tests for evolutionary trees and other dendrograms. Proc Natl Acad Sci USA 91:12293–12297
    [Google Scholar]
  17. Burgdorfer W., Brinton L. P., Krinsky W. L., Philip R. N. 1978; Rickettsia rhipicephali: a new spotted fever group rickettsia from the brown dog tick Rhipicephalus sanguineus. In Rickettsiae and Rickettsial Diseases pp. 307–316 Edited by Kazar J., Ormsbee R. A., Tarasevich I. N. Bratislava: Publishing House of the Slovak Academy of Sciences;
    [Google Scholar]
  18. Burgdorfer W., Aeschlimann A., Peter O., Hayes S. F., Philip R. N. 1979; Ixodes ricinus: vector of a hitherto undescribed spotted fever group agent in Switzerland. Acta Trop 39:357–367
    [Google Scholar]
  19. Chen D. Q., Campbell B. C., Purcell A. H. 1996; A new rickettsia from a herbivorous insect, the peaaphid Acyrthosiphon pisum (Harris). Curr Microbiol 33:123–128
    [Google Scholar]
  20. Crocquet-Valdes P. A., Weiss K., Walker D. H. 1994; Sequence analysis of the 190-kDa antigen-encoding gene of Rickettsia conorii (Malish strain). Gene 140:115–119
    [Google Scholar]
  21. Dessen P., Fondrat C., Valencien C., Munier G. 1990; BISANCE: a French service for access to biomolecular sequences databases. Cabios 6:355–356
    [Google Scholar]
  22. Drancourt M., Kelly P. J., Regnery R., Raoult D. 1992; Identification of spotted fever group rickettsiae using polymerase chain reaction and restriction-endonuclease length polymorphism analysis. Acta Virol 36:1–6
    [Google Scholar]
  23. Eremeeva M. E., Beati L., Makarova V. A., Fetisova N. F., Tarasevich I. V., Balayeva N. M., Raoult D. 1994a; Astrakhan fever rickettsiae: antigenic and genotypic analysis of isolates obtained from human and Rhipicephalus pumilio ticks. Am J Trop Med Hyg 51:697–706
    [Google Scholar]
  24. Eremeeva M., Yu X., Raoult D. 1994b; Differentiation among spotted fever group rickettsiae species by analysis of restriction fragment length polymorphism of PCR-amplified DNA. J Clin Microbiol 32:803–810
    [Google Scholar]
  25. Eremeeva M., Balayeva N., Roux V., Ignatovitch V., Kotsinjan M., Raoult D. 1995; Genomic and protein characterization of strain S, a rickettsia isolated from Rhipicephalus sanguineus ticks in Armenia. J Clin Microbiol 33:2738–44
    [Google Scholar]
  26. Felsenstein J. 1989; PHYLiP-phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  27. Gilmore R. D. Jr, Cieplak W. Jr, Policastro P. F., Hackstadt T. 1991; The 120 kilodalton outer membrane protein (OmpB) of Rickettsia rickettsii is encoded by an unusually long open reading frame: evidence for protein processing from a large precursor. Mol Microbiol 5:2361–2370
    [Google Scholar]
  28. Goldwasser R. A., Steiman Y., Klinberg W., Swartz T. A., Klinberg M. A. 1974; The isolation of strains of rickettsiae of the spotted fever group in Israel and their differentiation from other members of the group by immunofluorescence methods. Scand J Infect Dis 6:53–62
    [Google Scholar]
  29. Golinevitch H. 1960; A propos de la differentiation de quelques rickettsies du groupe de la fievre pourpree a tiques. Arch Inst Pasteur Tunis 37:13–22
    [Google Scholar]
  30. Hookey J. V., Bryden J., Gatehouse L. 1993; The use of 16S rDNA sequence analysis to investigate the phylogeny of Leptospiraceae and related spirochaetes. J Gen Microbiol 139:2585–2590
    [Google Scholar]
  31. Huebner R. J., Armstrong C. 1946; Rickettsialpox - a newly recognized rickettsial disease. I. Isolation of the etiological agent. Public Health Rep 61:1605
    [Google Scholar]
  32. Hughes A. L. 1991; Circumsporozoi'te protein genes of malaria parasites (Plasmodium spp.): evidence for positive selection on immunogenic regions. Genetics 127:345–363
    [Google Scholar]
  33. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  34. Kelly P. J., Beati L., Matthewman L. A., Mason P. R., Dasch G. A., Raoult D. 1994; A new pathogenic new spotted fever group rickettsia from Africa. J Trop Med Hyg 97:129–137
    [Google Scholar]
  35. Kimura M. 1980; A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
    [Google Scholar]
  36. Kumar S., Tamura K., Nei M. 1993; MEGA: molecular evolutionary genetics analysis, version 1.01. Pennsylvania State University; University Park:
    [Google Scholar]
  37. Marrero M., Raoult D. 1989; Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am J Trop Med Hyg 40:197–199
    [Google Scholar]
  38. Martinez-Murcia A. J., Benlloch S., Collins M. D. 1992; Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. Int J Syst Bacteriol 42:412–421
    [Google Scholar]
  39. Nei M., Gojobori T. 1986; Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol 3:418–426
    [Google Scholar]
  40. Olsen G. J., Woese C. R. 1993; Ribosomal RNA: a key to phylogeny. FASEB J 7:113–123
    [Google Scholar]
  41. Philip R. N., Casper E. A., Burgdorfer W, Gerloff R. K., Hugues L. E., Bell E. J. 1978; Serologic typing of rickettsiae of the spotted fever group by microimmunofluorescence. J Immunol 121:1961–1968
    [Google Scholar]
  42. Philip R. N., Casper E. A., Anacker R. L., Cory J., Hayes S. F., Burgdorfer W., Yunker C. E. 1983; Rickettsia bellii sp. nov.: tick-borne rickettsia, widely distributed in the United States, that is distinct from the spotted fever and typhus biogroups. Int J Syst Bacteriol 33:94–106
    [Google Scholar]
  43. Raoult D., Roux V. 1997; Rickettsioses as paradigms of new or emerging infectious diseases. Clin Microbiol Rev 10:694–719
    [Google Scholar]
  44. Regnery R. L., Spruill C. L., Plikaytis B. D. 1991; Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. Bacteriol 173:1576–1589
    [Google Scholar]
  45. Rehacek J. 1984; Rickettsia slovaca, the organism and its ecology. Acta Sci Nat Acad Bohemoslov Brno 18:1–50
    [Google Scholar]
  46. Rikihisa Y., Kawahara M., Wen B., Kociba G., Fuerst P., Kawamori F., Suto C., Shibata S., Futohahi M. 1997; Western immunoblot analysis of Haemobartonella muris and comparison of 16S rRNA sequences of H. muris, H. felis, and Eperythrozoon suis. J Clin Microbiol 35:823–829
    [Google Scholar]
  47. Robertson R. G., Wisseman C. L. Jr 1972; Tick-borne rickettsiae of the spotted fever group in West Pakistan. II. Serological classification of isolates from West Pakistan and Thailand: evidence for two new species. Am J Epidemiol 97:55–64
    [Google Scholar]
  48. Roux V., Raoult D. 1995; Phylogenetic analysis of the genus Rickettsia by 16S rDNA sequencing. Res Microbiol 146:385–396
    [Google Scholar]
  49. Roux V., Rydkina E., Eremeeva M., Raoult D. 1996a; Citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the rickettsiae. Int J Syst Bacteriol 47:252–261
    [Google Scholar]
  50. Roux V., Fournier P.-E., Raoult D. 1996b; Identification of the spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR amplified DNA of the gene encoding the protein rOmpA. J Clin Microbiol 34:2058–2065
    [Google Scholar]
  51. Roux V., Bergoin M., Lamaze N., Raoult D. 1997; Reassessment of the taxonomic position of Rickettsiella grylli. Int J Syst Bacteriol 47:1255–1257
    [Google Scholar]
  52. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4406–425
    [Google Scholar]
  53. Smith N. H., Maynard Smith J., Spratt B. G. 1995; Sequence evolution of the porB gene of Neisseria gonorrhoeae and Neisseria meningitidis: evidence of positive Darwinian selection. Mol Biol Evol 12:363–376
    [Google Scholar]
  54. Stothard D. R., Fuerst P. A. 1995; Evolutionary analysis of the spotted fever and typhus groups of Rickettsia using 16S rRNA gene sequences. Syst Appl Microbiol 18:52–61
    [Google Scholar]
  55. Sumner J. W., Sims K. G., Jones D. C., Anderson B. E. 1995; Protection of guinea-pigs from experimental Rocky Mountain spotted fever by immunization with baculovirus-expressed Rickettsia rickettsii rOmpA protein. Vaccine 13:29–35
    [Google Scholar]
  56. Tamura A., Ohashi N., Urakami H., Miyamura S. 1995; Classification of Rickettsia tsutsugamushi in a new genus, Orientia gen. nov., as Orientia tsutsugamushi comb. nov. Int J Syst Bacteriol 45:589–591
    [Google Scholar]
  57. Uchida T„, Uchiyama T., Kumano K., Walker D. H. 1992; Rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in Japan. Int J Syst Bacteriol 42:303–305
    [Google Scholar]
  58. Vishwanath S. 1991; Antigenic relationships among the rickettsiae of the spotted fever and typhus groups. FEMS Microbiol Lett 65:341–344
    [Google Scholar]
  59. Vishwanath S., McDonald G. A., Watkins N. G. 1990; A recombinant Rickettsia conorii vaccine protects guinea pigs from experimental boutonneuse fever and Rocky Mountain spotted fever. Infect Immun 58:646–653
    [Google Scholar]
  60. Walker D. H., Liu Q. H., Yu X. J., Li H., Taylor C., Feng H. M. 1992; Antigenic diversity of Rickettsia conorii. Am J Trop Med Hyg 47:78–86
    [Google Scholar]
  61. Weisburg W. G., Dobson M. E„, Samuel J. E. 7 others 1989; Phylogenetic diversity of the rickettsiae. J Bacteriol 171:4202–4206
    [Google Scholar]
  62. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  63. Weiss E., Moulder J. W. 1984; Order I. Rickettsiales Gieszczkiewicz 1939, 25AL, p. 687-701. In Bergey s Manual of Systematic Bacteriology vol. 1 pp. 687–703 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  64. Werren J. H., Hurst G. D., Zhang W., Breeuwer I. A. J., Stouthamer R., Majerus M. E. N. 1994; Rickettsial relative associated with male killing in the Ladybird Beetle (Adalia bipunctata). J Bacteriol 176:388–394
    [Google Scholar]
  65. Wilson A. C., Carlson S. S., White T. J. 1977; Biochemical evolution. Annu Rev Biochem 46:573–639
    [Google Scholar]
  66. Woese C. R. 1987; Bacterial evolution. Microbiol Rev 51:221–271
    [Google Scholar]
  67. Yu X., Jin Y., Fan M., Xu G., Liu Q., Raoult D. 1993; Genotypic and antigenic identification of two new strains of spotted fever group rickettsiae isolated from China. J Clin Microbiol 31:83–88
    [Google Scholar]
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