1887

Abstract

PML is a protein associated with discrete spherical structures within the nucleus of normal cells. A defect in PML expression is observed in acute promyelocytic leukaemia as a consequence of a chromosomal translocation involving the genes encoding PML and the α retinoic acid receptor (RARα). Disruption of PML bodies also occurs during herpes simplex virus infection after the immediate early protein Vmw110 has become associated with PML bodies. In this study, we followed the fate of PML bodies in human fibroblasts during the course of a human cytomegalovirus (CMV) infection. Disruption of PML bodies was observed to be dependent on CMV gene expression and to occur within 4 h post-infection, concomitant with the onset of CMV IE gene expression. Although a transient increase in the number of PML bodies could be observed in some cells, PML exists predominantly as a diffuse nuclear protein during both the early and late stages of CMV infection. Although the function of PML bodies is still uncertain, their disruption may be important for efficient herpes virus replication.

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/content/journal/jgv/10.1099/0022-1317-76-11-2887
1995-11-01
2024-04-26
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References

  1. Ascoli C. A., Maul G. G. 1991; Identification of a novel nuclear domain. Journal of Cell Biology 112:785–795
    [Google Scholar]
  2. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchinson C. A. III, Kouzarides T., Martignetti J. A., Preddie E., Satchwell S. C., Tomlinson P., Weston K. M., Barrell B. 1990; Analysis of the protein coding content and sequence of human cytomegalovirus strain AD169. Current Topics in Microbiology and Immunology 154:125–169
    [Google Scholar]
  3. Dyck J. A., Maul G. G., Miller W. H., Chen J. D., Kakizuka A., Evans R. M. 1994; A novel macromolecular structure is a target of the promyelocyte-retinoic acid receptor oncoprotein. Cell 76:333–343
    [Google Scholar]
  4. Everett R. D., Maul G. G. 1994; HSV-1 IE protein Vmw110 causes redistribution of PML. EMBO Journal 13:5062–5069
    [Google Scholar]
  5. Grignani F., Fagioli M., Ferrucci P. F., Alcalay M., Pelicci P. G. 1993; The molecular genetics of acute promyelocytic leukaemia. Blood Renews 7:87–93
    [Google Scholar]
  6. Koken M. M. H., Puvion-Dutilleul F., Guillemin M. C., Viron A., Linares-Cruz G., Stuurman N., De Jong L., Szostecki C., Calvo F., Chomienne C., Degos L., Puvion E., De The H. 1994; The T(15:17) translocation alters a nuclear body in a retinoic acid-reversible fashion. EMBO Journal 13:1073–1083
    [Google Scholar]
  7. Maul G. G., Everett R. D. 1994; The nuclear location of PML, a cellular member of the C3HC4 zinc-binding domain protein family, is rearranged during herpes simplex virus infection by the C3HC4 viral protein ICP0. Journal of General Virology 75:1223–1233
    [Google Scholar]
  8. Maul G. G., Guldner H. H., Spivack J. G. 1993; Modification of discrete nuclear domains induced by herpes simplex virus type 1 immediate early gene 1 product (ICP0). Journal of General Virology 74:2679–2690
    [Google Scholar]
  9. Minton E. J., Tysoe C., Sinclair J. H., Sissons J. G. P. 1994; Human cytomegalovirus infection of the monocyte/macrophage lineage in bone marrow. Journal of Virology 68:4017–4021
    [Google Scholar]
  10. Stasiak P. C., Mocarski E. S. 1992; Transactivation of the cytomegalovirus ICP36 gene promoter requires the a gene product TRS1 in addition to IE1 and IE2. Journal of Virology 66:1050–1058
    [Google Scholar]
  11. Stow E. C., Stow N. D. 1989; Complementation of a herpes simplex virus type 1 Vmwll0 deletion mutant by human cytomegalovirus. Journal of General Virology 70:695–704
    [Google Scholar]
  12. Stuurman N., De Graaf A., Floore A., Josso A., Humbel B., De Jong L., Van Driel R. 1992; A monoclonal antibody recognizing nuclear matrix-associated nuclear bodies. Journal of Cell Science 101:773–784
    [Google Scholar]
  13. Weston K. 1988; An enhancer element in the short unique region of human cytomegalovirus regulates the production of a group of abundant immediate early transcripts. Virology 162:406–416
    [Google Scholar]
  14. Wilkinson G. W. G., Akrigg A. 1992; Constitutive and enhanced expression from the CMV major IE promoter in a defective adenovirus vector. Nucleic Acids Research 20:2233–2239
    [Google Scholar]
  15. Wilkinson G. W. G., Akrigg A., Greenaway P. J. 1984; Transcription of the immediate early genes of human cytomegalovirus strain AD169. Virus Research 1:101–116
    [Google Scholar]
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