1887

Abstract

Two strains of previously unknown Gram-stain-positive, anaerobic, coccus-shaped bacteria from human wound specimens were characterized using phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies and distinguishable biochemical characteristics demonstrated that these two unknown strains, WAL 1855C and WAL 2038E, are genotypically homogeneous and constitute a novel lineage within cluster XIII. There was 13–14 % 16S rRNA gene sequence divergence between the novel strains and the most closely related species, , and species of . Based on the phenotypic and phylogenetic findings, a novel genus and species, gen. nov., sp. nov., are proposed. Strain WAL 1855C (=ATCC BAA-1631 =CCUG 55976) is the type strain of .

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2010-05-01
2024-04-26
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References

  1. Benson D. A., Boguski M. S., Lipman D. J., Ostell J. 1997; GenBank. Nucleic Acids Res 25:1–6 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Facklam R. R., Rodrigues U. M., Ruoff K. L. 1993; Phylogenetic analysis of some Aerococcus -like organisms from clinical sources: description of Helcococcus kunzii gen. nov., sp. nov. Int J Syst Bacteriol 43:425–429 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [CrossRef]
    [Google Scholar]
  4. Collins M. D., Falsen E., Foster G., Monasterio L. R., Dominguez L., Fernandez-Garazabal J. F. 1999; Helcococcus ovis sp. nov., a Gram-positive organism from sheep. Int J Syst Bacteriol 49:1429–1432 [CrossRef]
    [Google Scholar]
  5. Collins M. D., Falsen E., Brownlee K., Lawson P. A. 2004; Helcococcus sueciensis sp. nov., isolated from a human wound. Int J Syst Evol Microbiol 54:1557–1560 [CrossRef]
    [Google Scholar]
  6. Downes J., Wade W. G. 2006; Peptostreptococcus stomatis sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 56:751–754 [CrossRef]
    [Google Scholar]
  7. Ezaki T., Kawamura Y., Li N., Li Z. Y., Zhao L., Shu S. 2001; Proposal of the genera Anaerococcus gen.nov., Peptoniphilus gen. nov. and Gallicola gen. nov.for members of the genus Peptostreptococcus . Int J Syst Evol Microbiol 51:1521–1528
    [Google Scholar]
  8. Finegold S. M. 1977 Anaerobic Bacteria in Human Disease New York: Academic Press;
    [Google Scholar]
  9. Finegold S. M., Song Y., Liu C. 2002; Taxonomy – general comments and update on taxonomy of Clostridia and anaerobic cocci. Anaerobe 8:283–285 [CrossRef]
    [Google Scholar]
  10. Hooper S. J., Crean S. J., Lewis M. A., Spratt D. A., Wade W. G., Wilson M. J. 2006; Viable bacteria present within oral squamous cell carcinoma tissue. J Clin Microbiol 44:1719–1725 [CrossRef]
    [Google Scholar]
  11. Jousimies-Somer H., Summanen P., Citron D. M., Baron E. J., Wexler H. M., Finegold S. M. 2002 Wadsworth–KTL Anaerobic Bacteriology Manual , 6th edn. Belmont, CA: Star Publishing;
    [Google Scholar]
  12. Murdoch D. A. 1998; Gram-positive anaerobic cocci. Clin Microbiol Rev 11:81–120
    [Google Scholar]
  13. Song Y., Liu C., Mc Teague M., Finegold S. M. 2003; 16S ribosomal DNA sequence-based analysis of clinically significant Gram-positive anaerobic cocci. J Clin Microbiol 41:1363–1369 [CrossRef]
    [Google Scholar]
  14. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  15. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
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