1887

Abstract

The species is a promiscuous legume nodulated by several species of the family . During a study of rhizobia nodulating this legume in Portugal, we isolated several strains that nodulate effectively and also and , but they form ineffective nodules in . According to phylogenetic analysis of the 16S rRNA gene sequence, the strains from this study belong to the genus , with and as the closest related species, with 99.9 and 99.2 % similarity, respectively, between the type strains of these species and strain P1-7. The and genes carried by strain P1-7 are phylogenetically related to those of other species nodulating . This strain does not carry virulence genes present in the type strain of , ATCC 11325. Analysis of the and genes confirms this phylogenetic arrangement, showing low similarity with respect to those of ATCC 11325 (91.9 and 94.1 % similarity, respectively) and IIB CIAT 899 (90.6 % and 91.8 % similarity, respectively). The intergenic spacer (ITS) of the strains from this study is phylogenetically divergent from those of ATCC 11235 and CIAT 899, with 85.9 and 82.8 % similarity, respectively, with respect to strain P1-7. The tRNA profile and two-primer random amplified polymorphic DNA pattern of strain P1-7 are also different from those of ATCC 11235 and CIAT 899. The strains isolated in this study can be also differentiated from and by several phenotypic characteristics. The results of DNA–DNA hybridization showed means of 28 and 25 % similarity between strain P1-7 and ATCC 11235 and CIAT 899, respectively. All these data showed that the strains isolated in this study belong to a novel species of the genus , for which we propose the name sp. nov.; the type strain is P1-7 (=LMG 22705=CECT 7016).

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2006-11-01
2024-03-28
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