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Volume 58, Issue 6

Experimental infection in intratracheally inoculated mice Free

Abstract

This study established an experimental model of replicative infection in A/J mice. The animals were infected by intratracheal inoculation of 10–10 c.f.u. serogroup 1 (USA clinical isolates D4968, D4969 and D4973). The inocula of 10, 10, 10 and 10 c.f.u. of all tested serogroup 1 isolates were lethal for A/J mice. Inoculation of 10 c.f.u. caused death in 90 % of the animals within 5 days, whilst inoculation of 10 c.f.u. caused sporadic death of mice. All animals that received 10 c.f.u. bacteria developed acute lower respiratory disease, but were able to clear from the lungs within 3 weeks. The kinetics of bacterial growth in the lungs was independent of inoculum size and reached a growth peak about 3 logarithms above the initial inoculum at 72 h after inoculation. The most prominent histological changes in the lungs were observed at 48–72 h after inoculation in the form of a focal, neutrophil-dominant, peribronchiolar infiltration. The inflammatory process did not progress towards the interstitial or alveolar spaces. Immunohistological analyses revealed serogroup 1 during the early phase of infection near the bronchiolar epithelia and later co-localized with inflammatory cells. BALB/c and C57BL/6 mice strains were also susceptible to infection with all serogroup 1 strains tested and very similar changes were observed in the lungs of infected animals. These results underline the infection potential of serogroup 1, which is associated with high morbidity and lethality in mice.

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2009-06-01
2024-04-19
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Experimental Legionella longbeachae infection in intratracheally inoculated mice
J. Med. Microbiol. 58, 723 (2009); https://doi.org/10.1099/jmm.0.007476-0
/content/journal/jmm/10.1099/jmm.0.007476-0
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