The transcriptional activity of human Chromosome 22

  1. John L. Rinn1,2,5,
  2. Ghia Euskirchen1,5,
  3. Paul Bertone1,2,5,
  4. Rebecca Martone1,
  5. Nicholas M. Luscombe2,
  6. Stephen Hartman1,
  7. Paul M. Harrison2,
  8. F. Kenneth Nelson2,
  9. Perry Miller3,
  10. Mark Gerstein2,
  11. Sherman Weissman4, and
  12. Michael Snyder1,2,6
  1. 1Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103, USA; 2Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8114, USA; 3Department of Medical Anesthesiology, Yale University, New Haven, Connecticut 06520-8051, USA; 4Department of Genetics, Yale University, New Haven, Connecticut 06520-8005, USA

Abstract

A DNA microarray representing nearly all of the unique sequences of human Chromosome 22 was constructed and used to measure global-transcriptional activity in placental poly(A)+ RNA. We found that many of the known, related and predicted genes are expressed. More importantly, our study reveals twice as many transcribed bases as have been reported previously. Many of the newly discovered expressed fragments were verified by RNA blot analysis and a novel technique called differential hybridization mapping (DHM). Interestingly, a significant fraction of these novel fragments are expressed antisense to previously annotated introns. The coding potential of these novel expressed regions is supported by their sequence conservation in the mouse genome. This study has greatly increased our understanding of the biological information encoded on a human chromosome. To facilitate the dissemination of these results to the scientific community, we have developed a comprehensive Web resource to present the findings of this study and other features of human Chromosome 22 at http://array.mbb.yale.edu/chr22.

Keywords

Footnotes

  • 5 These authors contributed equally to this work.

  • 6 Corresponding author.

  • E-MAIL michael.snyder{at}yale.edu; FAX (203) 432-3597.

  • Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.1055203.

    • Received October 31, 2002.
    • Accepted December 24, 2002.
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