Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex

  1. Peter W. Lewis1,
  2. Eileen L. Beall1,
  3. Tracey C. Fleischer2,
  4. Daphne Georlette1,
  5. Andrew J. Link2, and
  6. Michael R. Botchan1,3
  1. 1Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA; 2Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA

Abstract

The Drosophila Myb complex has roles in both activating and repressing developmentally regulated DNA replication. To further understand biochemically the functions of the Myb complex, we fractionated Drosophila embryo extracts relying upon affinity chromatography. We found that E2F2, DP, RBF1, RBF2, and the Drosophila homolog of LIN-52, a class B synthetic multivulva (synMuv) protein, copurify with the Myb complex components to form the Myb-MuvB complex. In addition, we found that the transcriptional repressor protein, lethal (3) malignant brain tumor protein, L(3)MBT, and the histone deacetylase, Rpd3, associated with the Myb-MuvB complex. Members of the Myb-MuvB complex were localized to promoters and were shown to corepress transcription of developmentally regulated genes. These and other data now link together the Myb and E2F2 complexes in higher-order assembly to specific chromosomal sites for the regulation of transcription.

Keywords

Footnotes

  • Article published online ahead of print. Article and publication date are at http://www.genesdev.org/cgi/doi/10.1101/gad.1255204.

  • ↵3 Corresponding author. E-MAIL mbotchan{at}uclink4.berkeley.edu; FAX (510) 643-1729.

    • Accepted October 8, 2004.
    • Received August 27, 2004.
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This Article

  1. Published in Advance November 15, 2004, doi: 10.1101/gad.1255204 Genes & Dev. 18: 2929-2940 Cold Spring Harbor Laboratory Press

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