PBAF chromatin-remodeling complex requires a novel specificity subunit, BAF200, to regulate expression of selective interferon-responsive genes
- Zhijiang Yan1,
- Kairong Cui2,
- Darryl M. Murray1,
- Chen Ling1,
- Yutong Xue1,
- Amy Gerstein3,
- Ramon Parsons3,
- Keji Zhao2, and
- Weidong Wang1,4
- 1Laboratory of Genetics, National Institute on Aging, Baltimore, Maryland 21224, USA; 2Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA; 3Institute for Cancer Genetics, Columbia University, New York, New York 10032, USA
Abstract
PBAF and BAF are two chromatin-remodeling complexes of the SWI/SNF family essential for mammalian transcription and development. Although these complexes share eight identical subunits, only PBAF can facilitate transcriptional activation by nuclear receptors in vitro. Here we show that these complexes have selectivity in mediating transcription of different interferon-responsive genes. The selectivity by PBAF requires a novel subunit, BAF200, but not the previously described PBAF-specificity subunit, BAF180 (Polybromo). Our study provides in vivo evidence that PBAF and BAF regulate expression of distinct genes, and suggests that BAF200 plays a key role in PBAF function.
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Footnotes
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Supplemental material is available at http://www.genesdev.org.
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Article published online ahead of print. Article and publication date are at http://www.genesdev.org/cgi/doi/10.1101/gad.1323805.
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↵4 Corresponding author.
↵4 E-MAIL wangw{at}grc.nia.nih.gov; FAX (410) 558-8331.
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- Accepted May 27, 2005.
- Received April 15, 2005.
- Cold Spring Harbor Laboratory Press
