Human telomerase and Cajal body ribonucleoproteins share a unique specificity of Sm protein association
Abstract
Cajal bodies are nuclear structures that host RNA modification and assembly reactions. Some RNAs transit Cajal bodies, while others must concentrate in Cajal bodies to function. Here we report that at least a subfraction of human telomerase RNA and individual resident Cajal body RNAs is associated with Sm proteins. Surprisingly, of seven Sm proteins assembled into a heteroheptameric ring, only a subset copurifies telomerase and Cajal body ribonucleoproteins. We show that a Cajal body RNA localization motif determines this specificity. These discoveries expand the cellular repertoire of Sm protein assemblies and their involvement in ribonucleoprotein localization and function.
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Footnotes
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Supplemental material is available at http://www.genesdev.org.
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Article published online ahead of print. Article and publication date are at http://www.genesdev.org/cgi/doi/10.1101/gad.1390306.
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↵1 Corresponding author. E-MAIL kcollins{at}berkeley.edu; FAX (510) 643-6334.
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- Accepted December 28, 2005.
- Received November 4, 2005.
- Cold Spring Harbor Laboratory Press